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首页> 外文期刊>Journal of Environmental Science and Health. A, Toxic/Hazardous Substances & Environmental Engineering >Selection and Identification of Fungi Isolated from Sugarcane Bagasse and their Application for Phenanthrene Removal from Soil
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Selection and Identification of Fungi Isolated from Sugarcane Bagasse and their Application for Phenanthrene Removal from Soil

机译：甘蔗渣分离真菌的筛选，鉴定及其在土壤中除菲的应用

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摘要

This work investigated the identification and selection of fungi isolated from sugarcane bagasse and their application for phenanthrene (Phe) removal from soil. Fungi were identified by PCR amplification of ITS regions as Aspergillus terrus, Aspergillus fumi-gatus and Aspergillus niger, Penicillium glabrum and Cladosporium cladosporioides. A primary selection of fungi was accomplished in plate, considering Phe tolerance of every strain in two different media: potato dextrose agar (PDA) and mineral medium (MM). The radial extension rate (r_r) in PDA exhibited significant differences (p < 0.05) at 200 and 400 ppm of Phe. A secondary selection of A. niger, C. cladosporoides, and P. glabrum sp. was achieved based on their tolerance to 200, 400, 600 and 800 ppm of Phe, in solid culture at a sugarcane bagasse/contaminated soil ratio of 95:5, in Toyamas, Czapeck and Wunder media. Under these conditions, a maximum (70%) Phe removal by A. niger was obtained. In addition C. cladosporioides and A. niger were able to remove high (800 ppm) Phe concentrations.
机译：这项工作调查了从甘蔗渣中分离的真菌的鉴定和选择及其在土壤中去除菲（Phe）的应用。通过ITS区域的PCR扩增鉴定真菌为土曲霉，烟曲霉和黑曲霉，古生青霉和克氏梭状芽孢杆菌。考虑到每种菌株在两种不同培养基（马铃薯葡萄糖琼脂（PDA）和矿物培养基（MM））中的Phe耐受性，在平板中完成了真菌的主要选择。 PDA中的径向延伸率（r_r）在200和400 ppm的Phe上表现出显着差异（p <0.05）。次生选择黑曲霉，C. cladosporoides和P. glabrum sp。在Toyamas，Czapeck和Wunder培养基中，在甘蔗渣/受污染土壤比例为95：5的固态培养中，由于它们对200、400、600和800 ppm Phe的耐受性而实现了这一目标。在这些条件下，黑曲霉对苯丙氨酸的去除量最大（70％）。此外，刺孢梭菌和黑曲霉能够去除高浓度（800 ppm）的Phe。

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