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首页> 外文期刊>Journal of Environmental Science and Health. A, Toxic/Hazardous Substances & Environmental Engineering >Denitrification and Chemotaxis of Pseudomonas Stutzeri KC in Porous Media
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Denitrification and Chemotaxis of Pseudomonas Stutzeri KC in Porous Media

机译：多孔介质中假单胞菌KC的反硝化和趋化性

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Chemotaxis is an important mechanism by which microorganisms are dispersed in porous media. A vigorous chemotactic response to concentration gradients formed by microbial consumption of chemoattractants can accelerate transport of bacteria to highly contaminated regions of soils and sediments, enhancing the efficiency of in situ bioremediation operations. Although chemotaxis plays a key role in establishment of biodegradation zones in the subsurface, the effects of physical heterogeneity on bacterial motility are poorly understood. To investigate the influence of porous media heterogeneity on microbial chemotaxis, swarm plate migration experiments were conducted using Pseudomonas stutzeri strain KC, a denitrifying bacterium used for in situ biodegradation of carbon tetrachloride in groundwater. Swarm plate measurements indicate that strain KC is strongly chemotactic toward both acetate and nitrate. A three-component mathematical model was developed to describe the migration of strain KC. Estimates of chemotactic sensitivity were obtained in the homogeneous (agar) phase and in a heterogeneous medium of aquifer solids extracted from the Schoolcraft bioremediation field site in western Michigan. Interestingly, the motility of strain KC is significantly larger in the porous medium than in the aqueous phase. We hypothesize that chemotactic response is enhanced within the heterogeneous medium because chemoattractant gradients formed by nitrate consumption are larger in the confined spaces of the porous medium than in unconfined agar solution.
机译：趋化性是微生物分散在多孔介质中的重要机制。对微生物消耗化学趋化剂形成的浓度梯度的强烈趋化反应可加速细菌向土壤和沉积物高度污染区域的运输，从而提高原位生物修复操作的效率。尽管趋化性在地下生物降解区的建立中起着关键作用，但人们对物理异质性对细菌运动性的影响知之甚少。为了研究多孔介质异质性对微生物趋化性的影响，使用斯图氏假单胞菌KC菌群进行了平板迁移实验，该菌是一种用于地下水中四氯化碳原位生物降解的反硝化细菌。群体平板测量表明，菌株KC对乙酸盐和硝酸盐均具有强烈的趋化性。建立了一个由三部分组成的数学模型来描述菌株KC的迁移。趋化敏感性的估计是在均质（琼脂）相和从密歇根州西部的Schoolcraft生物修复场现场提取的含水层固体的非均质介质中获得的。有趣的是，在多孔介质中，菌株KC的运动性明显大于在水相中。我们假设异质培养基中的趋化反应得到增强，因为在多孔培养基的密闭空间中，硝酸盐消耗形成的趋化梯度比未封闭琼脂溶液大。

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