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首页> 外文期刊>Journal of Environmental Science and Health. A, Toxic/Hazardous Substances & Environmental Engineering >The Use of DNA Microsatellite Loci for 'Caretta Caretta' Identification
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The Use of DNA Microsatellite Loci for 'Caretta Caretta' Identification

机译：DNA微卫星基因座在'Caretta Caretta'鉴定中的应用

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Caretta carettas, one of the members of Chelonidae family, live primarily in water, except the periods of their ovulation where they come out to the shores to lay their eggs. Following an incubation period of 50-60 days, the newborns return to sea water to continue their 10-12-year life. Studies using marking methodology of the animals have shown that females return to the same place for ovulation every 2-3 years. The development of molecular genetic studies gives us now opportunity to trace all these routes that Caretta carettas follow during their life cycle. One of the basic techniques that is used for identification in general is the polymorphic DNA microsatellite loci. These 2-4 base pair DNA segments are considered to be ideal for Caretta caretta identification also. In this study we tried to establish a protocol in order to identify both male and female carettas in tissue samples collected from animals in the Mediterranean shoreline in the southern Turkey once this shoreline is one of the main spots of them for ovulation. The sampling has been done from 89 locations (Dalyan, Dalaman, Fethiye, Patara, Kale, Kumluca, Belek, Kizilot, Demirtas, Gazipasa and Anamur) from 246 dead baby Caretta carettas. DNA was extracted using silica-based extraction technology from the tissue homogenates. Cc7 locus was selected for identification to be tested for its degree of polymorphic content and therefore power of discrimination in general. The methodology used is PCR, followed by polyacrilamide gel electrophoresis and silver staining.
机译：Caretta carettas是Chelonidae家族的成员之一，主要生活在水中，除了排卵期，他们排到岸边产卵。经过50-60天的潜伏期，新生儿返回海水中继续其10-12岁的寿命。使用动物的标记方法进行的研究表明，雌性每2-3年返回同一地点进行排卵。分子遗传学研究的发展为我们提供了追踪Caretta carettas在其生命周期中遵循的所有这些途径的机会。通常用于识别的基本技术之一是多态性DNA微卫星基因座。这些2-4个碱基对的DNA片段也被认为是Caretta caretta鉴定的理想选择。在这项研究中，我们试图建立一个协议，以鉴定从土耳其南部地中海沿岸动物采集的组织样本中的雄性和雌性car蝶，一旦该海岸线成为它们排卵的主要场所之一。从246头死去的Caretta carettas的89个地点（Dalyan，Dalaman，Fethiye，Patara，羽衣甘蓝，Kumluca，Belek，Kizilot，Demirtas，Gazipasa和Anamur）进行了采样。使用基于二氧化硅的提取技术从组织匀浆中提取DNA。选择Cc7基因座进行鉴定，以测试其多态性含量的程度，因此通常可进行判别。所使用的方法是PCR，然后进行聚丙烯酰胺凝胶电泳和银染。

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