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首页> 外文期刊>Journal of Environmental Science and Health. A, Toxic/Hazardous Substances & Environmental Engineering >Determination of Geno- and Cytotoxicity of Groundwater and Sediments Using the Recombinant SWITCH Test
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Determination of Geno- and Cytotoxicity of Groundwater and Sediments Using the Recombinant SWITCH Test

机译：重组SWITCH试验测定地下水和沉积物的基因和细胞毒性

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摘要

The recombinant Salmonella typhimurium TA1535 strain carrying the SWITCH plasmid (combined construct of the SOS-Lux plasmid pPLS-1 and the LAC-Fluoro plasmid pGFPuv: pSWITCH) was treated with control substances for genotoxic (2-aminoanthracene) and cytotoxic (aureomycin) potency as well as with 18 environmental samples (groundwater, river water, sediments) provided at the SENSPOL Technical Meeting on Problems Related to Diffuse Pollution Sources (Characterization of Sediment, Dredged Material, and Groundwater) organized by the Federal Institute of Hydrology in Koblenz, Germany, during late October 2003. For metabolic activation the samples were treated with S9 mix (5% S9 fraction in cofactor mix, Aroclor 1254-induced rat liver microsomes, Moltox Inc., Mol, Belgium). Simultaneously determined cytotoxicity and genotoxicity data were derived through the Multilabel Counter 1420 Victor~2 (PerkinElmer, Boston, MA, USA), by sequential measurement of luminescence, absorbance, and fluorescence. The newly designed SWITCH test, as it was applied at the Koblenz meeting, displays a comparable sensitivity for test samples with known cyto-and genotoxic potential. Groundwater samples from the wells at the former gas plant site Kiel Canal and the agricultural area at Niederwerth expressed neither genotoxic nor cytotoxic responses of the bacteria for both metabolic conditions (+/—S9). Spiked groundwater samples from the Niederwerth well BW1 and the Urmitz well U12 located on the river Rhine were identified to be positive in terms of genotoxicity for the direct and the metabolic approach. Samples from the lake Tiefer See in the city of Potsdam showed a reduction of GFPuv expression as an indication for cytotoxicity, while luminescence output of incubated bacteria remained unaffected.
机译：携带SWITCH质粒（SOS-Lux质粒pPLS-1和LAC-Fluoro质粒pGFPuv：pSWITCH的组合构建体）的重组鼠伤寒沙门氏菌TA1535菌株用遗传毒性（2-氨基蒽）和细胞毒性（金霉素）效力的对照物质进行处理。以及在德国科布伦茨联邦水文研究所组织的SENSPOL关于与扩散污染源（沉积物，疏ged材料和地下水的特征化）有关的问题的SENSPOL技术会议上提供的18个环境样品（地下水，河水，沉积物）在2003年10月下旬期间。为进行代谢活化，将样品用S9混合物（辅因子混合物中5％的S9馏分，Aroclor 1254诱导的大鼠肝微粒体，Moltox Inc.，Mol，比利时）处理。同时测定的细胞毒性和遗传毒性数据是通过Multilabel Counter 1420 Victor-2（PerkinElmer，波士顿，马萨诸塞州，美国）通过顺序测量发光，吸光度和荧光而得出的。在科布伦茨会议上应用的新设计的SWITCH测试对具有已知细胞和遗传毒性潜力的测试样品显示出可比的灵敏度。在两种新陈代谢条件下，从前天然气厂基尔运河的井中的地下水样品和尼德韦斯的农业地区的地下水样品均未表现出细菌的遗传毒性和细胞毒性反应（+/- S9）。在直接和代谢方法的遗传毒性方面，从位于莱茵河上的Niederwerth BW1井和Urmitz U12井的掺入地下水样品经鉴定为阳性。来自波茨坦市的蒂弗湖（Tiefer See）湖的样品显示GFPuv表达降低，表明其具有细胞毒性，而培养细菌的发光输出未受影响。

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