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首页> 外文期刊>Journal of Environmental Science and Health. A, Toxic/Hazardous Substances & Environmental Engineering >Reductive Dechlorination of Tetrachloroethene by a Mixed Bacterial Culture Growing on Ethyl Lactate
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Reductive Dechlorination of Tetrachloroethene by a Mixed Bacterial Culture Growing on Ethyl Lactate

机译：乳酸乙酯上混合细菌培养物对四氯乙烯的还原脱氯作用

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Chloroethenes like tetrachloroethene (PCE) are the most prevalent groundwater contaminants in the USA. Their presence as nonaqueous phase liquids (NAPLs) makes remediation difficult. Among options for NAPL cleanup, co-solvent injection has demonstrated success. However, the process has the potential to leave considerable residue of the co-solvent as well as residual chloroethene. Our rationale in this study was to examine whether this residual solvent could be a potential electron donor for the remediation of the residual chloroethene. We hypothesized that ethyl lactate, a “green” solvent, could serve both as a NAPL extraction solvent and an electron donor for reductive dechlorination of residual chloroethene. We examined whether a mixed culture known to degrade PCE with lactate could also grow on ethyl lactate and whether it could stimulate PCE dechlorination. Biomass growth and PCE dechlorination were observed by protein and chloride production, respectively, in the culture; with a specific dechlorination rate of 50–150 μg (mg cell d)~(-1). Ethyl lactate abiotically breaks down to ethanol and lactate, the latter being a rich source of hydrogen for reductive dechlorination. The results demonstrate that ethyl lactate may be promising for in situ bioremediation following NAPL extraction.
机译：像四氯乙烯（PCE）这样的氯乙烯是美国最普遍的地下水污染物。它们作为非水相液体（NAPL）的存在使修复变得困难。在NAPL净化选项中，助溶剂注入已证明是成功的。但是，该方法有可能留下大量的助溶剂残留物和残留的氯乙烯。在这项研究中，我们的基本原理是检查这种残留溶剂是否可以作为补救残留氯乙烯的潜在电子供体。我们假设乳酸乙酯（一种“绿色”溶剂）既可以用作NAPL提取溶剂，又可以用作残留氯乙烯还原脱氯的电子给体。我们检查了一种已知的降解乳酸的PCE的混合培养物是否也可以在乳酸乙酯上生长，以及它是否可以刺激PCE脱氯。通过培养物中蛋白质和氯化物的产生分别观察到生物量的生长和PCE脱氯；特定脱氯速率为50–150μg（mg cell d）〜（-1）。乳酸乙酯非生物分解为乙醇和乳酸，后者是还原性脱氯的丰富氢气来源。结果表明，乳酸乙酯在NAPL提取后有望用于原位生物修复。

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