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首页> 外文期刊>Journal of Environmental Science and Health. A, Toxic/Hazardous Substances & Environmental Engineering >Disparate outer membrane exclusionary properties underlie intrinsic resistance to hydrophobic substances in Pseudomonas spp. isolated from surface waters under triclosan selection
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Disparate outer membrane exclusionary properties underlie intrinsic resistance to hydrophobic substances in Pseudomonas spp. isolated from surface waters under triclosan selection

机译：不同的外膜排除特性基于疏水性物质的特性抗性疏水性物质。 从Triclosan选择下的地表水隔离

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摘要

Representative members of surface water microbiota were obtained from three unrelated municipal sites in Oklahoma by direct plating under selection by the hydrophobic biocide triclosan. Multiple methods were employed to determine if intrinsic triclosan resistance reflected resistance to hydrophobic molecules by virtue of outer membrane impermeability. While all but one organism isolated in the absence of triclosan were able to initiate growth on MacConkey agar, only one was able to initiate significant growth with triclosan present. In contrast, all bacteria selected with triclosan were identified as Pseudomonas spp. using 16S RNA gene sequencing and exhibited growth comparable to Pseudomonas aeruginosa controls in the presence of hydrophobic antibacterial agents to include triclosan. Two representative bacteria isolated in the absence of triclosan allowed for greater outer membrane association with the fluorescent hydrophobic probe 1-N-phenylnapthylamine than did two triclosan-resistant isolates. Compound 48/80 disruption of outer membrane impermeability properties for hydrophobic substances either partially or fully sensitized nine of twelve intrinsically resistant isolates to triclosan. These data suggest that outer membrane exclusion underlies intrinsic resistance to triclosan in some, but not all Pseudomonas spp. isolated by selection from municipal surface waters and implicates the involvement of concomitant triclosan resistance mechanisms.
机译：通过疏水杀生物剂三氯烷的选择，通过直接电镀从俄克拉荷马州的三个无关的市政场地获得地表水微生物的代表性成员。采用多种方法来确定借助于外膜不可渗透性的固有三氯烷抗性反射对疏水分子的抗性。虽然孤立在没有三氯烷的某种生物体中，但能够引发Macconkey琼脂的生长，只有一个能够用三氯烷表现出现显着的增长。相比之下，用三氯烷选择的所有细菌被鉴定为假单胞菌SPP。使用16S RNA基因测序并表现出与疏水性抗菌剂存在的假单胞菌铜绿假单胞菌对照的增长，以包括三氯烷。在没有三氯烷的情况下隔离的两种代表性细菌允许与荧光疏水探针1-N-苯基萘胺的更大外膜结合而不是两种三氯菊酯隔离物。化合物48/80对疏水性物质的外膜不渗透性质的破坏部分或完全敏感九个与三氯烷的十二个本质上抗性分离物。这些数据表明，外膜排除在某些情况下为三胞软糖体潜入特性抗性，但并非所有的假单胞菌SPP。通过从城市水域的选择分离，并暗示伴随三胞胎抗性机制的参与。

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