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首页> 外文期刊>Journal of Environmental Sciences >Biological formation of 5-aminolevulinic acid by photosynthetic bacteria
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Biological formation of 5-aminolevulinic acid by photosynthetic bacteria

机译：光合细菌对5-氨基乙酰丙酸的生物形成

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In this study, 7 stains of Rhodopseudomonas sp. were selected from 36 photosynthetic bacteria stains storied in our laboratory. Rhodopseudomonas sp. strain 99-28 has the highest 5-aminolevulinic acid(ALA) production ability in these 7 strains. Rhodopseudomonas sp. 99-28 strain was mutated using ultraviolet radiation and a mutant strain L-l, which ALA production is higher than wild strain 99-28 about one times, was obtained. The elements affecting ALA formation of strain 99-28 and L-l were studied. Under the optimal condition(pH 7.5, supplement of ALA dehydratase(ALAD) inhibitor, levulinic acid(LA) and precursors of ALA synthesis, glycine and succinat, 3000 Ix of light density), ALA formation of mutant L-l was up to 22.15 mg/L. Strain L-l was used to treat wastewater to remove COD_(Cr) and produce ALA. ALA production was 2.819 mg/L, 1.531 mg/L, 2.166 mg/L, and 2.424 mg/L in monosodium glutamate wastewater (MOW), succotash wastewater(SW), brewage wastewater(BW), and citric acid wastewater (CAW) respectively. More than 90 percent of COD_(Cr) was removed in four kinds of wastewater. When LA, glycin and succinate were supplied, ALA production was dramatically increased, however, COD_(Cr) could hardly be removed.
机译：在这项研究中，Rhodopseudomonas sp。的7种染色。从我们实验室储存的36种光合细菌染色中选择。红假单胞菌在这7个菌株中，菌株99-28具有最高的5-氨基乙酰丙酸（ALA）生产能力。红假单胞菌使用紫外线辐射使99-28菌株突变，并获得突变菌株L-1，其ALA产量比野生菌株99-28高约一倍。研究了影响菌株99-28和L-1的ALA形成的元素。在最佳条件下（pH 7.5，补充ALA脱水酶（ALAD）抑制剂，乙酰丙酸（LA）和ALA合成的前体，甘氨酸和琥珀酸，3000 Ix的光密度），突变体L1的ALA形成高达22.15 mg / L. L-1菌株用于处理废水以去除COD_（Cr）并产生ALA。谷氨酸钠废水（MOW），蔗糖废水（SW），酿造废水（BW）和柠檬酸废水（CAW）中的ALA产量分别为2.819 mg / L，1.531 mg / L，2.166 mg / L和2.424 mg / L分别。在四种废水中去除了超过90％的COD_（Cr）。当提供LA，甘氨酸和琥珀酸酯时，ALA的产量显着增加，但是，COD_（Cr）几乎不能去除。

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