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首页> 外文期刊>Journal of Environmental Sciences >Variations of dominant microbiai populations in groundwater in response to the leachate from Laogang Landfill
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Variations of dominant microbiai populations in groundwater in response to the leachate from Laogang Landfill

机译:老港垃圾填埋场渗滤液对地下水微生物种群的影响

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摘要

Temporal changes of dominant microbial populations in groundwater in response to the leachate from Shanghai Laogang Landfill were investigated. Concentrations of dissolved redox- relevant species in groundwater suggested that the dominating redox process had changed from denitrification to methane- production/sulfate- reduction due to landfilling. Dominant microbial populations were determined using restriction fragment length polymorphism(RFLP) analyses of 16S rRNA gene libraries, which were further studied by sequencing and phylogenetic analyses. The results indicated that obvious shifts of dominant microbial populations had occurred in groundwater in response to the pollution of leachate. The closest relatives of some dominant clones are accordant with the dominating redox processes determined by hydrochemical analyses, based on the GenBank's indications on the ability to perform redox reactions.
机译:研究了上海老港垃圾填埋场渗滤液对地下水中主要微生物种群的时间变化。地下水中溶解的与氧化还原相关的物种的浓度表明,由于填埋,主要的氧化还原过程已从反硝化变为甲烷生成/硫酸盐还原。使用16S rRNA基因文库的限制性片段长度多态性(RFLP)分析确定了优势微生物种群,并通过测序和系统发育分析对其进行了进一步研究。结果表明,由于渗滤液的污染,地下水中的优势微生物种群发生了明显的变化。根据GenBank关于执行氧化还原反应能力的指示,一些优势克隆的最接近的亲戚与通过水化学分析确定的主要氧化还原过程一致。

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