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首页> 外文期刊>Journal of Environmental Sciences >DNA damage of germ cell of rat induced by nitrotoluene chemicals
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DNA damage of germ cell of rat induced by nitrotoluene chemicals

机译:硝基甲苯化学物诱导的大鼠生殖细胞DNA损伤

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摘要

2,4-dinitrotoluene(2,4-DNT), 2,6-dinitrotoluene(2,6-DNT) and 4-nitrotoluene(4-NT) are typical pollutants in the Songhua River of Northeast China. Sertoli/germ cell cocultures and single cell gel electrophoresis(SCGE) are applied to investigate whether they have genotoxicity on DNA damage of germ cell of Kunming male rat. The results showed that all three nitrotoluene compounds tested could induce DNA single-strand breaks of the germ cell. A significant relationship is found between logarithm dose and the degree of DNA damage, which implies that 2,4-DNT, 2,6-DNT and 4-NT have genotoxicity and can induce the germ cell DNA strand to break in vitro.
机译:2,4-二硝基甲苯(2,4-DNT),2,6-二硝基甲苯(2,6-DNT)和4-硝基甲苯(4-NT)是东北松花江的典型污染物。应用支持细胞/生殖细胞共培养和单细胞凝胶电泳(SCGE)研究它们对昆明雄性大鼠生殖细胞DNA损伤是否具有遗传毒性。结果表明,测试的所有三种硝基甲苯化合物都可以诱导生殖细胞的DNA单链断裂。发现对数剂量与DNA损伤程度之间存在显着关系,这表明2,4-DNT,2,6-DNT和4-NT具有遗传毒性,并可以诱导生殖细胞DNA链在体外断裂。

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