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首页> 外文期刊>Journal of Experimental Botany >TaNAC2, a NAC-type wheat transcription factor conferring enhanced multiple abiotic stress tolerances in Arabidopsis
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TaNAC2, a NAC-type wheat transcription factor conferring enhanced multiple abiotic stress tolerances in Arabidopsis

机译：TaNAC2，一种NAC型小麦转录因子，可增强拟南芥中的多种非生物胁迫耐受性

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摘要

Environmental stresses such as drought, salinity, and cold are major factors that significantly limit agricultural productivity. NAC transcription factors play essential roles in response to various abiotic stresses. However, the paucity of wheat NAC members functionally characterized to date does not match the importance of this plant as a world staple crop. Here, the function of TaNAC2 was characterized in Arabidopsis thaliana. A fragment of TaNAC2 was obtained from suppression subtractive cDNA libraries of wheat treated with polyethylene glycol, and its full-length cDNA was obtained by searching a full-length wheat cDNA library. Gene expression profiles indicated that TaNAC2 was involved in response to drought, salt, cold, and abscisic acid treatment. To test its function, transgenic Arabidopsis lines overexpressing TaNAC2–GFP controlled by the cauliflower mosaic virus 35S promoter were generated. Overexpression of TaNAC2 resulted in enhanced tolerances to drought, salt, and freezing stresses in Arabidopsis, which were simultaneously demonstrated by enhanced expression of abiotic stress-response genes and several physiological indices. Therefore, TaNAC2 has potential for utilization in transgenic breeding to improve abiotic stress tolerances in crops.
机译：干旱，盐碱和寒冷等环境压力是严重限制农业生产力的主要因素。 NAC转录因子在应对各种非生物胁迫中起着至关重要的作用。然而，迄今为止，功能上缺乏特征的小麦NAC成员与该植物作为世界主要农作物的重要性不符。在此，在拟南芥中表征了TaNAC2的功能。从用聚乙二醇处理的小麦的抑制性消减cDNA文库获得TaNAC2的片段，并通过搜索全长小麦cDNA文库获得其全长cDNA。基因表达谱表明TaNAC2参与干旱，盐，冷和脱落酸处理的响应。为了测试其功能，产生了由花椰菜花叶病毒35S启动子控制的过表达TaNAC2-GFP的转基因拟南芥品系。 TaNAC2的过表达导致拟南芥对干旱，盐和冰冻胁迫的耐受性增强，非生物胁迫响应基因的表达增强和一些生理指标同时证明了这一点。因此，TaNAC2具有在转基因育种中利用的潜力，可以改善作物的非生物胁迫耐受性。

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