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首页> 外文期刊>Journal of Experimental Botany >The interaction of the novel 30C02 cyst nematode effector protein with a plant β-1,3-endoglucanase may suppress host defence to promote parasitism
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The interaction of the novel 30C02 cyst nematode effector protein with a plant β-1,3-endoglucanase may suppress host defence to promote parasitism

机译：新型30C02囊肿线虫效应蛋白与植物β-1,3-内切葡聚糖酶的相互作用可能抑制宿主防御从而促进寄生

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摘要

Phytoparasitic nematodes secrete an array of effector proteins to modify selected recipient plant cells into elaborate and essential feeding sites. The biological function of the novel 30C02 effector protein of the soybean cyst nematode, Heterodera glycines, was studied using Arabidopsis thaliana as host and the beet cyst nematode, Heterodera schachtii, which contains a homologue of the 30C02 gene. Expression of Hg30C02 in Arabidopsis did not affect plant growth and development but increased plant susceptibility to infection by H. schachtii. The 30C02 protein interacted with a specific (AT4G16260) host plant β-1,3-endoglucanase in both yeast and plant cells, possibly to interfere with its role as a plant pathogenesis-related protein. Interestingly, the peak expression of 30C02 in the nematode and peak expression of At4g16260 in plant roots coincided at around 3–5 d after root infection by the nematode, after which the relative expression of At4g16260 declined significantly. An Arabidopsis At4g16260 T-DNA mutant showed increased susceptibility to cyst nematode infection, and plants that overexpressed At4g16260 were reduced in nematode susceptibility, suggesting a potential role of host β-1,3-endoglucanase in the defence response against H. schachtii infection. Arabidopsis plants that expressed dsRNA and its processed small interfering RNA complementary to the Hg30C02 sequence were not phenotypically different from non-transformed plants, but they exhibited a strong RNA interference-mediated resistance to infection by H. schachtii. The collective results suggest that, as with other pathogens, active suppression of host defence is a critical component for successful parasitism by nematodes and a vulnerable target to disrupt the parasitic cycle.
机译：植物寄生线虫分泌一系列效应蛋白，以将选定的受体植物细胞修饰为精巧而必不可少的觅食位点。使用拟南芥作为宿主和甜菜孢囊线虫（Heterodera schachtii）研究了大豆孢囊线虫的新型30C02效应蛋白，其生物学功能，该甜菜孢囊线虫含有30C02基因的同源物。 Hg30CO 2在拟南芥中的表达不影响植物生长发育，但增加了植物对沙门氏菌感染的敏感性。 30CO2蛋白与特定的（AT4G16260）宿主植物β-1,3-内切葡聚糖酶在酵母和植物细胞中相互作用，可能会干扰其作为植物发病相关蛋白的作用。有趣的是，线虫感染根后，线虫中30CO2的峰值表达与植物根中At4g16260的峰值表达重合，大约在3-5天后，At4g16260的相对表达显着下降。拟南芥At4g16260 T-DNA突变体显示出对囊肿线虫感染的敏感性增加，而过表达At4g16260的植物的线虫敏感性降低，表明宿主β-1,3-内切葡聚糖酶在抵抗沙门氏菌感染的防御反应中具有潜在作用。表达dsRNA及其加工的与Hg30CO2序列互补的小干扰RNA的拟南芥植物与非转化植物在表型上没有差异，但它们表现出强大的RNA干扰介导的抗沙门氏菌感染能力。总体结果表明，与其他病原体一样，主动抑制宿主防御是线虫成功寄生的关键要素，也是破坏寄生虫循环的脆弱目标。

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