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首页> 外文期刊>Journal of Experimental Botany >Seven zinc-finger transcription factors are novel regulators of the stress responsive gene OsDREB1B
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Seven zinc-finger transcription factors are novel regulators of the stress responsive gene OsDREB1B

机译：七个锌指转录因子是应激反应基因OsDREB1B的新型调节剂

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Plants have evolved several mechanisms in order to cope with adverse environmental conditions. The transcription factors (TFs) belonging to the DREB1/CBF subfamily have been described as major regulators of the plant responses to different abiotic stresses. This study focused on the rice gene OsDREB1B, initially described as highly and specifically induced by cold. However, here it is shown that OsDREB1B is not only induced by low temperatures, but also by drought and mechanical stress. In order to identify novel TFs that bind to its promoter, a yeast one-hybrid system was used to screen a cold-induced cDNA expression library. Thereby seven novel Zn-finger TFs were identified that bind to the promoter of OsDREB1B. Among them, there were four Zn-finger homeodomain (ZF-HD) and three C2H2-type Zn-finger TFs. Gene expression studies showed that these TFs are differentially regulated at transcriptional level by different abiotic stress conditions, which is illustrative of the crosstalk between stress signalling pathways. Protein–protein interaction studies revealed the formation of homo- and heterodimers among the ZF-HD TFs identified, but not for the C2H2-type. Using a transactivation assay in Arabidopsis protoplasts, all the TFs identified repressed the expression of the reporter gene, driven by the promoter of OsDREB1B. This assay also showed that the dimerization observed between the ZF-HD TFs may play a role on their transactivation activity. The results here presented suggest a prominent role of Zn-finger TFs in the regulation of OsDREB1B.
机译：植物已经进化出几种机制来应对不利的环境条件。属于DREB1 / CBF亚科的转录因子（TFs）已被描述为植物对不同非生物胁迫的反应的主要调节因子。这项研究的重点是水稻基因OsDREB1B，最初被描述为由寒冷引起的高特异性诱导。但是，此处显示OsDREB1B不仅是由低温引起的，而且是由干旱和机械应力引起的。为了鉴定与其启动子结合的新型TF，使用酵母单杂交系统筛选冷诱导的cDNA表达文库。因此，鉴定了七个与OsDREB1B的启动子结合的新颖的锌指TF。其中，有四个锌指同源域（ZF-HD）和三个C 2 H 2 型锌指TFs。基因表达研究表明，这些TF在转录水平上受到不同的非生物胁迫条件的差异调节，这说明了胁迫信号通路之间的串扰。蛋白质-蛋白质相互作用研究表明，已鉴定的ZF-HD TF之间存在同二聚体和异二聚体，但C 2 H 2 型没有。使用拟南芥原生质体中的反式激活测定法，鉴定出的所有TF都抑制了由OsDREB1B启动子驱动的报告基因的表达。该测定法还表明，在ZF-HD TF之间观察到的二聚化可能对其反式激活活性起作用。此处显示的结果表明，锌指TF在OsDREB1B的调节中起着重要作用。

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