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首页> 外文期刊>Journal of Experimental Botany >D-type cyclins control cell division and developmental rate during Arabidopsis seed development
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D-type cyclins control cell division and developmental rate during Arabidopsis seed development

机译：D型细胞周期蛋白控制拟南芥种子发育过程中的细胞分裂和发育速率

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摘要

Seed development in Arabidopsis is characterized by stereotypical division patterns, suggesting that coordinated control of cell cycle may be required for correct patterning and growth of the embryo and endosperm. D-type cyclins (CYCD) are key cell cycle regulators with roles in developmental processes, but knowledge regarding their involvement in seed development remains limited. Here, a family-wide gene expression, and loss- and gain-of-function approach was adopted to reveal additional functions for CYCDs in the development of seed tissues. CYCD genes have both discrete and overlapping tissue-specific expression patterns in the seed as revealed by GUS reporter gene expression. Analysis of different mutant combinations revealed that correct CYCD levels are required in seed development. The CYCD3 subgroup is specifically required as its loss caused delayed development, whereas overexpression in the embryo and endosperm of CYCD3;1 or a previously uncharacterized gene, CYCD7;1, variously leads to induced proliferation, abnormal phenotypes, and elevated seed abortion. CYCD3;1 overexpression provoked a delay in embryonic developmental progression and abnormalities including additional divisions of the hypophysis and suspensor, regions where CYCD3 genes are normally expressed, but did not affect endosperm development. Overexpression of CYCD7;1, not normally expressed in seed development, promoted overgrowth of both embryo and endosperm through increased division and cell enlargement. In contrast to post-germination growth, where pattern and organ size is not generally related to division, results suggest that a close control of cell division through regulation of CYCD activity is important during seed development in conferring both developmental rate and correct patterning.
机译：拟南芥种子发育的特征是刻板印象的分型模式，这表明可能需要协调控制细胞周期才能正确地构图胚胎和胚乳。 D型细胞周期蛋白（CYCD）是关键的细胞周期调节剂，在发育过程中发挥作用，但有关其参与种子发育的知识仍然有限。在这里，采用了全家族的基因表达以及功能丧失和获得功能的方法来揭示CYCD在种子组织发育中的附加功能。 CYCD基因在种子中具有离散和重叠的组织特异性表达模式，如GUS报告基因表达所揭示。对不同突变体组合的分析表明，种子发育过程中需要正确的CYCD水平。特别需要CYCD3亚组，因为其丢失会导致发育延迟，而CYCD3; 1或以前未表征的基因CYCD7; 1在胚胎和胚乳中的过表达会导致诱导的增殖，异常表型和更高的种子流产率。 CYCD3; 1的过度表达引起胚胎发育进程的延迟和异常，包括垂体和悬垂物的额外分裂，即正常表达CYCD3基因但不影响胚乳发育的区域。 CYCD7; 1的过表达通常不会在种子发育中表达，它通过增加分裂和细胞扩大来促进胚胎和胚乳的过度生长。与发芽后生长相反，在发芽后生长中，模式和器官大小通常与分裂无关，结果表明，通过调节CYCD活性来密切控制细胞分裂在种子发育过程中对赋予发育速率和正确的构图都很重要。

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    《Journal of Experimental Botany》 |2012年第10期|p.3571-3586|共16页
  • 作者

    Carl Collins12†* Walter Dewitte13 and James A. H. Murray13*;

  • 作者单位

    1Institute of Biotechnology, University of Cambridge, Tennis Court Road, Cambridge, CB2 1QT, UK 2Natural Resources Institute, University of Greenwich, Central Avenue, Chatham Maritime, Kent, ME4 4TB, UK 3Cardiff School of Biosciences, Cardiff University, Museum Avenue, Cardiff, CF10 3AX, UK;

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