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首页> 外文期刊>Journal of Experimental Botany >Changes in the chloroplast proteome following water deficit and subsequent watering in a high- and a low-drought-tolerant genotype of Festuca arundinacea
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Changes in the chloroplast proteome following water deficit and subsequent watering in a high- and a low-drought-tolerant genotype of Festuca arundinacea

机译：高水分胁迫和低水分胁迫的Festuca arundinacea基因型缺水和随后浇水后叶绿体蛋白质组的变化

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Festuca arundinacea is one of the most drought-tolerant species within the Lolium–Festuca complex and was used as a model for research aimed at identifying the chloroplast components involved in the proteomic response for drought stress in forage grasses. Individual F. arundinacea genotypes with contrasting levels of drought tolerance, the high-drought-tolerant (HDT) and the low-drought-tolerant (LDT) genotypes, were selected for comparative physiological and proteomic work. Measurements of water uptake, chlorophyll fluorescence, relative water content, electrolyte leakage, and gas exchange during drought and rewatering periods were followed by investigations on accumulation levels of chloroplast proteins before drought conditions, on d 3 and 11 of drought treatment, and after 10 d of subsequent watering, using two-dimensional gel electrophoresis. The proteins that were accumulated differentially between the selected plants were then identified by mass spectrometry. The LDT genotype revealed lower levels of water uptake and relative water content as drought progressed, and this was accompanied by lower levels of transpiration and net photosynthesis, and a higher level of electrolyte leakage observed in this genotype. Eighty-two protein accumulation profiles were compared between the HDT and LDT genotypes and ten proteins were shown to be differentially accumulated between them. The functions of the selected proteins in plant cells and their probable influence on the process of recovery after drought treatment in F. arundinacea are discussed.
机译：Festuca arundinacea是黑麦草-Festuca复合物中最耐旱的物种之一，被用作研究模型，旨在鉴定草料草中干旱胁迫的蛋白质组学响应中涉及的叶绿体成分。选择具有相对不同水平的耐旱性，高耐旱性（HDT）和低耐旱性（LDT）基因型的单个金合子基因型进行比较的生理和蛋白质组学研究。在干旱和复水期间测量水分吸收，叶绿素荧光，相对水含量，电解质泄漏和气体交换，然后研究干旱条件之前，干旱处理第3天和第11天以及干旱后10天后叶绿体蛋白质的积累水平。随后的浇水，使用二维凝胶电泳。然后通过质谱鉴定在所选植物之间差异积累的蛋白质。 LDT基因型显示出随着干旱的进行，水分吸收和相对水分含量降低，并且伴随着蒸腾作用和净光合作用的降低，以及该基因型中电解质泄漏的升高。比较了HDT和LDT基因型之间的82种蛋白质积累概况，并显示了10种蛋白质在它们之间的差异积累。讨论了选择的蛋白质在植物细胞中的功能及其对干旱处理后金合欢（F. arundinacea）恢复过程的可能影响。

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