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首页> 外文期刊>Journal of Experimental Botany >New perspectives on glutamine synthetase in grasses
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New perspectives on glutamine synthetase in grasses

机译:谷氨酰胺合成酶的新观点

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Members of the glutamine synthetase (GS) gene family have now been characterized in many crop species such as wheat, rice, and maize. Studies have shown that cytosolic GS isoforms are involved in nitrogen remobilization during leaf senescence and emphasized a role in seed production particularly in small grain crop species. Data from the sequencing of genomes for model crops and expressed sequence tag (EST) libraries from non-model species have strengthened the idea that the cytosolic GS genes are organized in three functionally and phylogenetically conserved subfamilies. Using a bioinformatic approach, the considerable publicly available information on high throughput gene expression was mined to search for genes having patterns of expression similar to GS. Interesting new hypotheses have emerged from searching for co-expressed genes across multiple unfiltered experimental data sets in rice. This approach should inform new experimental designs and studies to explore the regulation of the GS gene family further. It is expected that understanding the regulation of GS under varied climatic conditions will emerge as an important new area considering the results from recent studies that have shown nitrogen assimilation to be critical to plant acclimation to high CO2 concentrations.
机译:谷氨酰胺合成酶(GS)基因家族的成员现已在许多农作物物种中得到了表征,例如小麦,水稻和玉米。研究表明,胞质GS同工型在叶片衰老过程中参与氮的转运,并强调了其在种子生产中的作用,特别是在小粒作物中。来自模型农作物的基因组测序数据和来自非模型物种的表达序列标签(EST)文库,进一步强化了将细胞溶质GS基因组织在三个功能和系统发育保守的亚家族中的观念。使用生物信息学方法,挖掘了大量有关高通量基因表达的公开可用信息,以搜索具有类似于GS的表达模式的基因。通过在水稻中多个未过滤的实验数据集中搜索共表达的基因,出现了有趣的新假设。这种方法应该为新的实验设计和研究提供参考,以进一步探索GS基因家族的调控。鉴于最近的研究表明氮同化对植物适应高CO 2 的浓度至关重要,因此,人们期望了解不同气候条件下GS的调控将成为一个重要的新领域。

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