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首页> 外文期刊>Journal of Experimental Botany >The cyclic nucleotide-gated channels AtCNGC11 and 12 are involved in multiple Ca2+-dependent physiological responses and act in a synergistic manner
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The cyclic nucleotide-gated channels AtCNGC11 and 12 are involved in multiple Ca2+-dependent physiological responses and act in a synergistic manner

机译：环状核苷酸门控通道AtCNGC11和12参与多个Ca 2 + 依赖性生理反应，并以协同方式起作用

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Arabidopsis cyclic nucleotide-gated ion channels (AtCNGCs) form a large family consisting of 20 members. These channels have so far been reported to be involved in a diverse range of physiological phenomena. For example, AtCNGC18 was reported to play an important role in pollen tube growth, while AtCNGC2, 4, 11, and 12 were implicated in mediating pathogen defence. To identify additional functions for AtCNGC11 and 12, various physiological aspects were analysed using both AtCNGC11 and 12 single knockout mutants as well as a double mutant. Although AtCNGC11 and 12 can function as K+ and Ca2+ channels in yeast, it was found that the loss of AtCNGC11 and 12 in Arabidopsis caused increased sensitivity to Ca2+ but not K+, indicating a specific function for these genes in Ca2+ signalling in planta. However, they did not show an alteration in Ca2+ accumulation, suggesting that AtCNGC11 and 12 are not involved in general Ca2+ homeostasis but rather in the endogenous movement of Ca2+ and/or Ca2+ signalling. Furthermore, these channels synergistically contribute to the generation of a Ca2+ signal that leads to gravitropic bending. Finally, AtCNGC11 and 12 gene expression was induced during dark-induced senescence and AtCNGC11 and 12 knockout mutants displayed enhanced chlorophyll loss, which was even more pronounced in the double mutant, also indicating synergistic roles in senescence. The findings indicate that (i) some CNGC family members have multiple physiological functions and (ii) some plant CNGCs share the same biological function and work in a synergistic manner.
机译：拟南芥环核苷酸门控离子通道（AtCNGCs）形成了一个由20个成员组成的大家族。迄今为止，已经报道这些通道涉及多种生理现象。例如，据报道AtCNGC18在花粉管生长中起重要作用，而AtCNGC2、4、11和12与介导病原体防御有关。为了确定AtCNGC11和12的其他功能，使用AtCNGC11和12个单敲除突变体以及一个双突变体对各种生理方面进行了分析。尽管AtCNGC11和12可以在酵母中充当K + 和Ca 2 + 通道，但是发现拟南芥中AtCNGC11和12的缺失导致对Ca 2 + ，而不是K + ，表明这些基因在植物体内Ca 2 + 信号中具有特定功能。但是，它们没有显示Ca 2 + 积累的变化，表明AtCNGC11和12不参与一般的Ca 2 + 稳态，而是参与Ca的内源性运动。 2 + 和/或Ca 2 + 信号。此外，这些通道协同作用有助于产生Ca 2 + 信号，从而导致重力弯曲。最后，在黑暗诱导的衰老过程中诱导了AtCNGC11和12基因的表达，AtCNGC11和12基因敲除突变体显示出增强的叶绿素损失，在双突变体中更明显，也表明了在衰老中的协同作用。这些发现表明：（i）一些CNGC家族成员具有多种生理功能，并且（ii）一些植物CNGC具有相同的生物学功能，并以协同方式起作用。

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