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首页> 外文期刊>Journal of Experimental Botany >QUASIMODO 3 (QUA3) is a putative homogalacturonan methyltransferase regulating cell wall biosynthesis in Arabidopsis suspension-cultured cells
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QUASIMODO 3 (QUA3) is a putative homogalacturonan methyltransferase regulating cell wall biosynthesis in Arabidopsis suspension-cultured cells

机译：QUASIMODO 3（QUA3）是拟南芥悬浮培养细胞中推测的高半乳糖醛酸甲基转移酶，调控细胞壁的生物合成

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摘要

Pectins are complex polysaccharides that are essential components of the plant cell wall. In this study, a novel putative Arabidopsis S-adenosyl-L-methionine (SAM)-dependent methyltransferase, termed QUASIMODO 3 (QUA3, At4g00740), has been characterized and it was demonstrated that it is a Golgi-localized, type II integral membrane protein that functions in methylesterification of the pectin homogalacturonan (HG). Although transgenic Arabidopsis seedlings with overexpression, or knock-down, of QUA3 do not show altered phenotypes or changes in pectin methylation, this enzyme is highly expressed and abundant in Arabidopsis suspension-cultured cells. In contrast, in cells subjected to QUA3 RNA interference (RNAi) knock-down there is less pectin methylation as well as altered composition and assembly of cell wall polysaccharides. Taken together, these observations point to a Golgi-localized QUA3 playing an essential role in controlling pectin methylation and cell wall biosynthesis in Arabidopsis suspension cell cultures.
机译：果胶是复杂的多糖，是植物细胞壁的重要组成部分。在这项研究中，一种新型的拟南芥S-腺苷-L-蛋氨酸（SAM）依赖的甲基转移酶QUASIMODO 3（QUA3，At4g00740）已被表征，并证明它是高尔基体定位的II型整体膜。在果胶高半乳糖醛酸聚糖（HG）的甲基酯化中起作用的蛋白质。尽管具有过量表达或敲除QUA3的转基因拟南芥幼苗没有表现出改变的表型或果胶甲基化变化，但该酶在拟南芥悬浮培养的细胞中高表达并丰富。相反，在遭受QUA3 RNA干扰（RNAi）敲除的细胞中，果胶甲基化程度降低，并且细胞壁多糖的组成和组装改变。综上所述，这些观察结果表明，在拟南芥悬浮细胞培养物中，高尔基体定位的QUA3在控制果胶甲基化和细胞壁生物合成中起着至关重要的作用。

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