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首页> 外文期刊>Journal of Experimental Botany >DNA alteration and programmed cell death during ageing of sunflower seed
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DNA alteration and programmed cell death during ageing of sunflower seed

机译：向日葵种子老化过程中的DNA改变和程序性细胞死亡

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摘要

Sunflower (Helianthus annuus L.) seed viability is affected by moisture content (MC) during ageing and is related to accumulation of hydrogen peroxide and changes in energy metabolism. The aim of the present work was to investigate the effect of ageing on DNA alteration events by RAPD (random amplification of polymorphic DNA) analysis and to determine whether loss of seed viability might correspond to a controlled programmed cell death (PCD). Ageing of sunflower seeds was carried out at 35 °C for 7 d at different MCs. The higher the MC, the lower was the seed viability. RAPD analysis showed that DNA alterations occurred during ageing especially in seeds containing a high MC. In addition, PCD, as revealed by DNA fragmentation and TUNEL (terminal deoxynucleotide transferase-mediated dUTP nick-end labelling) assay, was detected in aged seeds at MCs which resulted in ∼50% seed viability. At the cellular level, TUNEL assay and propidium iodide staining showed that cell death concerns all the cells of the embryonic axis. The quantification of the adenylate pool highlights mitochondrial dysfunction in aged seeds containing a high MC. The involvement of oxidative burst, mitochondria dysfunction, and PCD in seed loss of viability is proposed.
机译：向日葵（Helianthus annuus L.）种子的活力受衰老过程中水分含量（MC）的影响，并且与过氧化氢的积累和能量代谢的变化有关。本工作的目的是通过RAPD（多态性DNA的随机扩增）分析研究衰老对DNA改变事件的影响，并确定种子活力的丧失是否可能对应于受控程序性细胞死亡（PCD）。向日葵种子的老化在35°C下在不同MC进行7 d。 MC越高，种子活力越低。 RAPD分析表明，在老化过程中会发生DNA改变，尤其是在含有高MC的种子中。此外，DNA片段化和TUNEL（末端脱氧核苷酸转移酶介导的dUTP缺口末端标记）测定揭示了PCD，在MC的老化种子中检测到PCD，导致约50％的种子活力。在细胞水平上，TUNEL分析和碘化丙锭染色表明细胞死亡涉及胚胎轴的所有细胞。腺苷酸池的量化突出了含有高MC的老化种子中的线粒体功能障碍。提出了氧化爆发，线粒体功能障碍和PCD参与种子活力丧失。

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