...
  • 主题
高级搜索  >
历史搜索 清空历史
首页> 外文期刊>Journal of Experimental Botany >Isolation and characterization of low-sulphur-tolerant mutants of Arabidopsis
【24h】

Isolation and characterization of low-sulphur-tolerant mutants of Arabidopsis

机译：拟南芥低硫耐性突变体的分离与鉴定

获取原文
获取原文并翻译 | 示例
           
页面导航
  • 摘要
  • 著录项
  • 相似文献
  • 相关主题

摘要

Sulphur is an essential element for plant growth and development as well as for defence against biotic and abiotic stresses. Increasing sulphate utilization efficiency (SUE) is an important issue for crop improvement. Little is known about the genetic determinants of sulphate utilization efficiency. No gain-of-function mutants with improved SUE have been reported to date. Here the isolation and characterization of two low-sulphur-tolerant mutants, sue3 and sue4 are reported using a high-throughput genetic screen where a ‘sulphur-free’ solid medium was devised to give the selection pressure necessary to suppress the growth of the wild-type seedlings. Both mutants showed improved tolerance to low sulphur conditions and well-developed root systems. The mutant phenotype of both sue3 and sue4 was specific to sulphate deficiency and the mutants displayed enhanced tolerance to heavy metal and oxidative stress. Genetic analysis revealed that sue3 was caused by a single recessive nuclear mutation while sue4 was caused by a single dominant nuclear mutation. The recessive locus in sue3 is the previously identified VirE2-interacting Protein 1. The dominant locus in sue4 is a function-unknown locus activated by the four enhancers on the T-DNA. The function of SUE3 and SUE4 in low sulphur tolerance was confirmed either by multiple mutant alleles or by recapitulation analysis. Taken together, our results demonstrate that this genetic screen is a reasonable approach to isolate Arabidopsis mutants with improved low sulphur tolerance and potentially with enhanced sulphate utilization efficiency. The two loci identified in sue3 and sue4 should assist in understanding the molecular mechanisms of low sulphur tolerance.
机译：硫是植物生长和发育以及防御生物和非生物胁迫的重要元素。提高硫酸盐利用率（SUE）是改善作物的重要问题。关于硫酸盐利用效率的遗传决定因素知之甚少。迄今为止，尚未报道具有改善的SUE的功能获得突变体。在这里使用高通量遗传筛选报告了两个低硫耐性突变株sue3和sue4的分离和表征，其中设计了“无硫”固体培养基以提供抑制野生生长所需的选择压力。型幼苗。两种突变体均显示出对低硫条件和发达的根系的耐受性提高。 sue3和sue4的突变体表型都对硫酸盐缺乏具有特异性，并且突变体对重金属和氧化应激的耐受性增强。遗传分析显示sue3由单个隐性核突变引起，而sue4由单个显性核突变引起。 sue3中的隐性基因座是先前鉴定的VirE2相互作用蛋白1。sue4中的显性基因座是功能未知的基因座，由T-DNA上的四个增强子激活。通过多个突变体等位基因或通过归纳分析证实了SUE3和SUE4在低硫耐受性中的功能。两者合计，我们的结果表明，这种遗传筛选是一种分离拟南芥突变体的合理方法，该突变体具有改善的低硫耐受性并可能具有提高的硫酸盐利用效率。 sue3和sue4中鉴定出的两个基因座应有助于理解低硫耐受性的分子机制。

著录项

相似文献

  • 外文文献
  • 中文文献
  • 专利
获取原文

客服邮箱：kefu@zhangqiaokeyan.com

京公网安备：11010802029741号 ICP备案号：京ICP备15016152号-6 六维联合信息科技 (北京) 有限公司©版权所有

  • 客服微信

  • 服务号