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首页> 外文期刊>Journal of Experimental Botany >Arabidopsis dynamin-like protein DRP1A: a null mutant with widespread defects in endocytosis, cellulose synthesis, cytokinesis, and cell expansion
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Arabidopsis dynamin-like protein DRP1A: a null mutant with widespread defects in endocytosis, cellulose synthesis, cytokinesis, and cell expansion

机译：拟南芥动力蛋白样蛋白DRP1A：一种无效突变体，在胞吞作用，纤维素合成，胞质分裂和细胞扩增中具有广泛的缺陷

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摘要

Dynamin-related proteins are large GTPases that deform and cause fission of membranes. The DRP1 family of Arabidopsis thaliana has five members of which DRP1A, DRP1C, and DRP1E are widely expressed. Likely functions of DRP1A were identified by studying rsw9, a null mutant of the Columbia ecotype that grows continuously but with altered morphology. Mutant roots and hypocotyls are short and swollen, features plausibly originating in their cellulose-deficient walls. The reduction in cellulose is specific since non-cellulosic polysaccharides in rsw9 have more arabinose, xylose, and galactose than those in wild type. Cell plates in rsw9 roots lack DRP1A but still retain DRP1E. Abnormally placed and often incomplete cell walls are preceded by abnormally curved cell plates. Notwithstanding these division abnormalities, roots and stems add new cells at wild-type rates and organ elongation slows because rsw9 cells do not grow as long as wild-type cells. Absence of DRP1A reduces endocytotic uptake of FM4-64 into the cytoplasm of root cells and the hypersensitivity of elongation and radial swelling in rsw9 to the trafficking inhibitor monensin suggests that impaired endocytosis may contribute to the development of shorter fatter roots, probably by reducing cellulose synthesis.
机译：与动力蛋白有关的蛋白质是大的GTPases，它们会变形并引起膜裂变。拟南芥的DRP1家族有五个成员，其中广泛表达了DRP1A，DRP1C和DRP1E。通过研究rsw9可以确定DRP1A的功能，rsw9是哥伦比亚生态型的无效突变体，其连续生长但形态发生了变化。突变的根和下胚轴短而肿胀，其特征可能源自其缺乏纤维素的壁。纤维素的减少是特定的，因为rsw9中的非纤维素多糖比野生型具有更多的阿拉伯糖，木糖和半乳糖。 rsw9根中的细胞板缺少DRP1A，但仍保留DRP1E。异常放置且经常不完整的细胞壁之前是异常弯曲的细胞板。尽管存在这些分裂异常，但由于rsw9细胞的生长不如野生型细胞长，因此根和茎仍以野生型速率添加新细胞，并且器官伸长变慢。缺乏DRP1A会减少FM4-64进入根细胞胞质的内吞摄取，而rsw9的延长和放射状肿胀对贩运抑制剂莫能菌素的超敏性表明，内吞能力受损可能有助于较短脂肪根的发育，可能是通过减少纤维素合成。

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