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首页> 外文期刊>Journal of Experimental Botany >Quantitative expression analysis of the ABC genes in Sophora tetraptera, a woody legume with an unusual sequence of floral organ development
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Quantitative expression analysis of the ABC genes in Sophora tetraptera, a woody legume with an unusual sequence of floral organ development

机译：豆科植物豆科植物具有不同花序器官发育序列的ABC基因的定量表达分析

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Sophora is a woody genus of the Leguminosae in which an unusual order and process of floral organ development is often observed. The SEM results for Sophora tetraptera revealed precocious initiation of the carpel, delayed development of petals, and floral organ development interrupted by an unusual prolonged summer–autumn dormant period which occurred between organ initiation and organ differentiation. These observations provided an opportunity to track key floral identity genes over an extended developmental period. Homologues of LEAFY, APETALA1, PISTILLATA, and AGAMOUS were isolated from S. tetraptera. Real-time PCR enabled a simultaneous and quantitative analysis of both the temporal and spatial expression patterns of these four genes. Expression differences in the range of three to five orders of magnitude were detected between different genes and between different stages of flower development for the same gene. Although not functionally tested, the spatial expression patterns of the genes were consistent with expectations based on the ABC model of floral development. Their temporal expression patterns were consistent with the timing of flower initiation and the unusual order of organ development. Quantitatively, while the expression levels of the LFY homologue and the A-class gene were high during the periods of organ initiation and organ differentiation and low during the summer–autumn dormant period, high expression levels of the B- and C-class genes were detected only during the rapid, albeit delayed, phase of organ differentiation. Additionally, the sustained expression of the floral organ identity genes after differentiation reflects on-going roles for these genes during subsequent organ development.
机译：槐属是豆科的木本属，其中经常观察到花器官发育的异常顺序和过程。四倍体槐的扫描电镜结果表明，心皮早熟，花瓣发育迟缓，花器官发育被器官启动和器官分化之间异常延长的夏季-秋季休眠期打断。这些观察结果提供了在延长的发育时期内追踪关键花卉同一性基因的机会。 LEAFY，APETALA1，PISTILLATA和AGAMOUS的同系物是从四足链球菌中分离出来的。实时PCR能够同时定量分析这四个基因的时间和空间表达模式。在同一基因的不同基因之间以及花朵发育的不同阶段之间，检测到的表达差异在三到五个数量级之间。尽管未经功能测试，但基因的空间表达模式与基于花卉发育的ABC模型的预期一致。它们的时间表达模式与花开始的时间和器官发育的异常顺序一致。从数量上讲，虽然LFY同源基因和A类基因的表达水平在器官启动和器官分化期间较高，而在夏季至秋季的休眠期则较低，但B和C类基因的较高表达水平仅在器官分化的快速阶段（尽管延迟）才检测到。另外，花器官识别基因在分化后的持续表达反映了这些基因在随后的器官发育过程中的持续作用。

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