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首页> 外文期刊>Journal of Experimental Botany >Overproduction of C4 photosynthetic enzymes in transgenic rice plants: an approach to introduce the C4-like photosynthetic pathway into rice
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Overproduction of C4 photosynthetic enzymes in transgenic rice plants: an approach to introduce the C4-like photosynthetic pathway into rice

机译：转基因水稻植株中C 4 光合酶的过量生产：将C 4 样光合途径引入水稻的途径

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Four enzymes, namely, the maize C4-specific phosphoenolpyruvate carboxylase (PEPC), the maize C4-specific pyruvate, orthophosphate dikinase (PPDK), the sorghum NADP-malate dehydrogenase (MDH), and the rice C3-specific NADP-malic enzyme (ME), were overproduced in the mesophyll cells of rice plants independently or in combination. Overproduction individually of PPDK, MDH or ME did not affect the rate of photosynthetic CO2 assimilation, while in the case of PEPC it was slightly reduced. The reduction in CO2 assimilation in PEPC overproduction lines remained unaffected by overproduction of PPDK, ME or a combination of both, however it was significantly restored by the combined overproduction of PPDK, ME, and MDH to reach levels comparable to or slightly higher than that of non-transgenic rice. The extent of the restoration of CO2 assimilation, however, was more marked at higher CO2 concentrations, an indication that overproduction of the four enzymes in combination did not act to concentrate CO2 inside the chloroplast. Transgenic rice plants overproducing the four enzymes showed slight stunting. Comparison of transformants overproducing different combinations of enzymes indicated that overproduction of PEPC together with ME was responsible for stunting, and that overproduction of MDH had some mitigating effects. Possible mechanisms underlying these phenotypic effects, as well as possibilities and limitations of introducing the C4-like photosynthetic pathway into C3 plants, are discussed.
机译：玉米C 4 特异性磷酸烯醇丙酮酸羧化酶（PEPC），玉米C 4 特异性丙酮酸，正磷酸二激酶（PPDK），高粱NADP-苹果酸四种酶脱氢酶（MDH）和水稻C 3 特异的NADP-苹果酸酶（ME）在水稻植株的叶肉细胞中单独或联合过量生产。 PPDK，MDH或ME的过量生产并没有影响光合CO 2 的同化率，而PEPC则略有降低。 PEPC生产过剩生产线中CO 2 同化的降低仍不受PPDK，ME或两者的过量生产的影响，但PPDK，ME和MDH的联合生产过剩达到水平已明显恢复相当于或略高于非转基因水稻。但是，在较高的CO 2 浓度下，CO 2 同化作用的恢复程度更加明显，这表明四种酶组合的过量生产不会起到浓缩作用叶绿体中的CO 2 。过量生产四种酶的转基因水稻植株显示出轻微的发育迟缓。比较过量产生不同酶组合的转化体表明，PEPC与ME一起过度生产是发育迟缓的原因，而MDH的过度生产具有一定的缓解作用。探讨了这些表型效应的潜在机制，以及将C 4 样光合途径引入C 3 植物的可能性和局限性。

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