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首页> 外文期刊>Journal of Experimental Botany >Can the cold tolerance of C4 photosynthesis in Miscanthus×giganteus relative to Zea mays be explained by differences in activities and thermal properties of Rubisco?
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Can the cold tolerance of C4 photosynthesis in Miscanthus×giganteus relative to Zea mays be explained by differences in activities and thermal properties of Rubisco?

机译：卢比斯科活性和热学性质的差异能否解释芒草×长翅类中C 4 光合作用的耐寒性？

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The previous investigations show that the amount and activity of Rubisco appears the major limitation to effective C4 photosynthesis at low temperatures. The chilling-tolerant and bioenergy feedstock species Miscanthus×giganteus (M.×giganteus) is exceptionally productive among C4 grasses in cold climates. It is able to develop photosynthetically active leaves at temperatures 6 °C below the minimum for maize, and achieves a productivity even at 52° N that exceeds that of the most productive C3 crops at this latitude. This study investigates whether this unusual low temperature tolerance can be attributed to differences in the amount or kinetic properties of Rubisco relative to maize. An efficient protocol was developed to purify large amounts of functional Rubisco from C4 leaves. The maximum carboxylation activities (Vmax), activation states, catalytic rates per active site (Kcat) and activation energies (Ea) of purified Rubisco and Rubisco in crude leaf extracts were determined for M.×giganteus grown at 14 °C and 25 °C, and maize grown at 25 °C. The sequences of M.×giganteus Rubisco small subunit mRNA are highly conserved, and 91% identical to those of maize. Although there were a few differences between the species in the translated protein sequences, there were no significant differences in the catalytic properties (Vmax, Kcat, and Ea) for purified Rubisco, nor was there any effect of growth temperature in M.×giganteus on these kinetic properties. Extracted activities were close to the observed rates of CO2 assimilation by the leaves in vivo. On a leaf area basis the extracted activities and activation state of Rubisco did not differ significantly, either between the two species or between growth temperatures. The activation state of Rubisco in leaf extracts showed no significant difference between warm and cold-grown M.×giganteus. In total, these results suggest that the ability of M.×giganteus to be productive and maintain photosynthetically competent leaves at low temperature does not result from low temperature acclimation or adaptation of the catalytic properties of Rubisco.
机译：先前的研究表明，Rubisco的量和活性似乎是低温下有效C 4 光合作用的主要限制因素。耐寒和生物能源的原料物种芒草（Miscanthus×giganteus）（M.×giganteus）在寒冷气候下的C 4 草中具有极高的生产力。它能够在低于玉米最低温度6°C的温度下发育具有光合活性的叶片，即使在52°N的条件下，仍能达到该纬度下生产力最高的C 3 作物的生产力。这项研究调查了这种异常的低温耐受性是否可归因于Rubisco相对于玉米的数量或动力学特性的差异。建立了从C 4 叶中纯化大量功能性Rubisco的有效方案。最大羧化活性（V max ），活化态，每个活性位的催化速率（K cat ）和活化能（E a ）测定在粗叶提取物中纯化的Rubisco和Rubisco的M.×giganteus在14°C和25°C下生长，在25°C下生长的玉米。 M.×giganteus Rubisco小亚基mRNA的序列是高度保守的，与玉米序列具有91％的同一性。尽管物种之间在翻译的蛋白质序列中存在一些差异，但是在催化性质（V max ，K cat 和E a ）纯化的Rubisco，M。×giganteus的生长温度对这些动力学特性也没有任何影响。提取的活性接近叶片体内观察到的CO 2 同化率。以叶面积为基础，Rubisco的提取活性和活化状态在两个物种之间或在生长温度之间都没有显着差异。叶片提取物中Rubisco的活化状态在冷和热生长的M.×giganteus之间没有显着差异。总的来说，这些结果表明M.×giganteus在低温下具有生产能力和保持光合作用能力的叶片的能力不是由低温驯化或Rubisco的催化特性的适应性产生的。

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  • 来源
    《Journal of Experimental Botany》 |2008年第7期|p.1779-1787|共9页
  • 作者

    Dafu Wang12 Shawna L. Naidu2* Archie R. Portis Jr234 Stephen P. Moose13 and Stephen P. Long123†;

  • 作者单位

    1Institute for Genomic Biology, University of Illinois at Urbana-Champaign, Urbana, IL 61801, USA 2Department of Plant Biology, University of Illinois at Urbana-Champaign, Urbana, IL 61801, USA 3Department of Crop Sciences, University of Illinois at Urbana-Champaign, Urbana, IL 61801, USA 4US Department of Agriculture, Photosynthesis Research Unit, Agricultural Research Service, Urbana, IL 61801, USA;

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