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首页> 外文期刊>Journal of Experimental Botany >How stable isotopes may help to elucidate primary nitrogen metabolism and its interaction with (photo)respiration in C3 leaves
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How stable isotopes may help to elucidate primary nitrogen metabolism and its interaction with (photo)respiration in C3 leaves

机译：稳定同位素如何帮助阐明C 3 叶片的初级氮代谢及其与（光）呼吸的相互作用

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摘要

Intense efforts are currently devoted to elucidate the metabolic networks of plants, in which nitrogen assimilation is of particular importance because it is strongly related to plant growth. In addition, at the leaf level, primary nitrogen metabolism interacts with photosynthesis, day respiration, and photorespiration, simply because nitrogen assimilation needs energy, reductant, and carbon skeletons which are provided by these processes. While some recent studies have focused on metabolomics and genomics of plant leaves, the actual metabolic fluxes associated with nitrogen metabolism operating in leaves are not very well known. In the present paper, it is emphasized that 12C/13C and 14N/15N stable isotopes have proved to be useful tools to investigate such metabolic fluxes and isotopic data are reviewed in the light of some recent advances in this area. Although the potential of stable isotopes remains high, it is somewhat limited by our knowledge of some isotope effects associated with enzymatic reactions. Therefore, this paper should be viewed as a call for more fundamental studies on isotope effects by plant enzymes.
机译：当前致力于研究植物的代谢网络，其中氮同化特别重要，因为氮同化与植物的生长密切相关。另外，在叶片水平上，主要的氮代谢与光合作用，日呼吸和光呼吸相互作用，这仅仅是因为氮同化需要这些过程提供的能量，还原剂和碳骨架。尽管最近的一些研究集中在植物叶片的代谢组学和基因组学上，但与叶片中氮代谢相关的实际代谢通量还不是很清楚。本文强调了 12 C / 13 C和 14 N / 15 N稳定同位素已被证明是研究此类代谢通量的有用工具，并结合该领域的最新进展对同位素数据进行了综述。尽管稳定同位素的潜力仍然很高，但是由于我们对与酶促反应相关的某些同位素效应的了解，其稳定性受到了一定程度的限制。因此，本文应被视为呼吁对植物酶对同位素的影响进行更基础的研究。

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    《Journal of Experimental Botany》 |2008年第7期|p.1685-1693|共9页
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    Guillaume Tcherkez12* and Michael Hodges3;

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    1Plateforme Métabolisme-Métabolome, IFR 87, Bât. 630, Université Paris Sud-XI, F-91405 Orsay cedex, France 2Laboratoire d’écophysiologie végétale, CNRS UMR 8079, Bât. 362, Université Paris Sud-XI, F-91405 Orsay cedex, France 3Institut de Biotechnologie des Plantes, CNRS UMR 8618, Bât. 630, Université Paris Sud-XI, F-91405 Orsay cedex, France;

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