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首页> 外文期刊>Journal of Experimental Botany >Interaction study of MADS-domain proteins in tomato
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Interaction study of MADS-domain proteins in tomato

机译：番茄中MADS结构域蛋白的相互作用研究

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MADS-domain proteins are important transcription factors involved in many biological processes of plants. Interactions between MADS-domain proteins are essential for their functions. In tomato (Solanum lycopersicum), the number of MIKCc-type MADS-domain proteins identified has totalled 36, but a large-scale interaction assay is lacking. In this study, 22 tomato MADS-domain proteins were selected from six functionally important subfamilies of the MADS-box gene family, to create the first large-scale tomato protein interaction network. Compared with Arabidopsis and petunia (Petunia hybrida), protein interaction patterns in tomato displayed both conservation and divergence. The majority of proteins that can be identified as putative orthologues exhibited conserved interaction patterns, and modifications were mostly found in genes underlining traits unique to tomato. JOINTLESS and RIN, characterized for their roles in abscission zone development and fruit ripening, respectively, showed enlarged interaction networks in comparison with their Arabidopsis and petunia counterparts. Novel interactions were also found for members of the expanded subfamilies, such as those represented by AP1/FUL and AP3/PI MADS-domain proteins. In search for higher order complexes, TM5 was found to be the preferred bridge among the five SEP-like proteins. Additionally, 16 proteins with the MADS-domain removed were used to assess the role of the MADS-domain in protein–protein interactions. The current work provides important knowledge for further functional and evolutionary study of the MADS-box genes in tomato.
机译：MADS结构域蛋白是植物许多生物学过程中涉及的重要转录因子。 MADS结构域蛋白之间的相互作用对其功能至关重要。在番茄（Solanum lycopersicum）中，鉴定出的MIKC c 型MADS结构域蛋白总数为36，但缺乏大规模的相互作用测定法。在这项研究中，从MADS-box基因家族的六个功能重要的亚家族中选择了22种番茄MADS结构域蛋白，以创建第一个大规模的番茄蛋白相互作用网络。与拟南芥和矮牵牛（矮牵牛）相比，番茄中的蛋白质相互作用模式显示出保守性和发散性。可以鉴定为直向同源物的大多数蛋白质表现出保守的相互作用模式，并且修饰大多出现在强调番茄特有性状的基因中。 JOINTLESS和RIN分别以其在脱落区发育和果实成熟中的作用为特征，与拟南芥和矮牵牛相比，显示出扩大的相互作用网络。还发现了扩展亚家族成员的新型相互作用，例如以AP1 / FUL和AP3 / PI MADS域蛋白为代表的成员。在寻找更高阶的复合物时，TM5被发现是五个SEP样蛋白中的首选桥梁。此外，使用16种去除了MADS域的蛋白来评估MADS域在蛋白与蛋白相互作用中的作用。目前的工作为进一步研究番茄MADS-box基因的功能和进化提供了重要的知识。

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