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首页> 外文期刊>Journal of Experimental Botany >Alteration in the chloroplastic metabolism leads to ROS accumulation in pea plants in response to plum pox virus
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Alteration in the chloroplastic metabolism leads to ROS accumulation in pea plants in response to plum pox virus

机译：叶绿素代谢改变会导致豌豆植物中的ROS积累，从而应对李子痘病毒

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In this work, a recombinant plum pox virus (PPV, Sharka) encoding green fluorescent protein is used to study its effect on antioxidant enzymes and protein expression at the subcellular level in pea plants (cv. Alaska). PPV had produced chlorotic spots as well as necrotic spots in the oldest leaves at 13–15 d post-inoculation. At 15 d post-inoculation, PPV was present in the chlorotic and necrotic areas, as shown by the fluorescence signal produced by the presence of the green fluorescent protein. In the same areas, an accumulation of reactive oxygen species was noticed. Studies with laser confocal and electron microscopy demonstrated that PPV accumulated in the cytosol of infected cells. In addition, PPV infection produced an alteration in the chloroplast ultrastructure, giving rise to dilated thylakoids, an increase in the number of plastoglobuli, and a decreased amount of starch content. At 3 d post-inoculation, although no changes in the oxidative stress parameters were observed, an increase in the chloroplastic hydrogen peroxide levels was observed that correlated with a decrease in the enzymatic mechanisms involved in its elimination (ascorbate peroxidase and peroxidase) in this cell compartment. These results indicate that an alteration in the chloroplastic metabolism is produced in the early response to PPV. This oxidative stress is more pronounced during the development of the disease (15 d post-inoculation) judging from the increase in oxidative stress parameters as well as the imbalance in the antioxidative systems, mainly at the chloroplastic level. Finally, proteomic analyses showed that most of the changes produced by PPV infection with regard to protein expression at the subcellular level were related mainly to photosynthesis and carbohydrate metabolism. It seems that PPV infection has some effect on PSII, directly or indirectly, by decreasing the amount of Rubisco, oxygen-evolving enhancer, and PSII stability factor proteins. The results indicate that Sharka symptoms observed in pea leaves could be due to an imbalance in antioxidant systems as well as to an increased generation of reactive oxygen species in chloroplasts, induced probably by a disturbance of the electron transport chain, suggesting that chloroplasts can be a source of oxidative stress during viral disease development.
机译：在这项工作中，使用编码绿色荧光蛋白的重组李子痘病毒（PPV，Sharka）来研究其对豌豆植物（阿拉斯加州）亚细胞水平上抗氧化酶和蛋白质表达的影响。 PPV在接种后13-15 d在最老的叶片中产生了褪绿斑和坏死斑。接种后15天，PPV存在于绿藻和坏死区域，如绿色荧光蛋白的存在所产生的荧光信号所示。在同一地区，发现了活性氧的积累。激光共聚焦和电子显微镜的研究表明，PPV积累在被感染细胞的细胞质中。此外，PPV感染会导致叶绿体超微结构发生变化，从而导致类囊体膨胀，质体球数增加和淀粉含量降低。接种后第3天，虽然未观察到氧化应激参数的变化，但观察到氯塑料过氧化氢水平的升高与该细胞中消除其的酶机制（抗坏血酸过氧化物酶和过氧化物酶）的降低有关隔间。这些结果表明在对PPV的早期应答中产生了叶绿体代谢的改变。从氧化应激参数的增加以及抗氧化系统的失衡（主要在叶绿体水平）来看，这种氧化应激在疾病发展期间（接种后15天）更为明显。最后，蛋白质组学分析表明，PPV感染引起的亚细胞水​​平蛋白质表达的大部分变化主要与光合作用和碳水化合物代谢有关。似乎PPV感染通过减少Rubisco，放氧增强剂和PSII稳定因子蛋白的量直接或间接对PSII产生某些影响。结果表明，豌豆叶片中观察到的Sharka症状可能是由于抗氧化剂系统失衡以及叶绿体中活性氧的产生增加所致，这很可能是由于电子传输链的扰动引起的，表明叶绿体可能是一种病毒性疾病发展过程中的氧化应激源。

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