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首页> 外文期刊>Journal of Experimental Botany >OsBIRH1, a DEAD-box RNA helicase with functions in modulating defence responses against pathogen infection and oxidative stress
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OsBIRH1, a DEAD-box RNA helicase with functions in modulating defence responses against pathogen infection and oxidative stress

机译：OsBIRH1，一种DEAD-box RNA解旋酶，具有调节针对病原体感染和氧化应激的防御反应的功能

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摘要

DEAD-box proteins comprise a large protein family with members from all kingdoms and play important roles in all types of processes in RNA metabolism. In this study, a rice gene OsBIRH1, which encodes a DEAD-box RNA helicase protein, was cloned and characterized. The predicted OsBIRH1 protein contains a DEAD domain and all conserved motifs that are common characteristics of DEAD-box RNA helicases. Recombinant OsBIRH1 protein purified from Escherichia coli was shown to have both RNA-dependent ATPase and ATP-dependent RNA helicase activities in vitro. Expression of OsBIRH1 was activated in rice seedling leaves after treatment with defence-related signal chemicals, for example benzothiadiazole, salicylic acid, l-aminocyclopropane-1-carboxylic acid, and jasmonic acid, and was also up-regulated in an incompatible interaction between a resistant rice genotype and the blast fungus, Magnaporthe grisea. Transgenic Arabidopsis plants that overexpress the OsBIRH1 gene were generated. Disease resistance phenotype assays revealed that the OsBIRH1-overexpressing transgenic plants showed an enhanced disease resistance against Alternaria brassicicola and Pseudomonas syringae pv. tomato DC3000. Meanwhile, defence-related genes, for example PR-1, PR-2, PR-5, and PDF1.2, showed an up-regulated expression in the transgenic plants. Moreover, the OsBIRH1 transgenic Arabidopsis plants also showed increased tolerance to oxidative stress and elevated expression levels of oxidative defence genes, AtApx1, AtApx2, and AtFSD1. The results suggest that OsBIRH1 encodes a functional DEAD-box RNA helicase and plays important roles in defence responses against biotic and abiotic stresses.
机译：DEAD-box蛋白由来自各个王国的成员组成的一个大蛋白家族，在RNA代谢的所有过程中都起着重要作用。在这项研究中，克隆并鉴定了水稻基因OsBIRH1，该基因编码DEAD-box RNA解旋酶蛋白。预测的OsBIRH1蛋白包含DEAD结构域和DEAD-box RNA解旋酶共同特征的所有保守基序。从大肠杆菌中纯化的重组OsBIRH1蛋白在体外具有RNA依赖性ATPase和ATP依赖性RNA解旋酶活性。在与防御相关的信号化学品（例如苯并噻二唑，水杨酸，1-氨基环丙烷-1-羧酸和茉莉酸）处理后，OsBIRH1的表达在水稻幼苗叶片中被激活，并且在与草甘膦之间的不相容相互作用中也被上调。水稻基因型和稻瘟病菌，稻瘟病菌。产生了过表达OsBIRH1基因的转基因拟南芥植物。疾病抗性表型分析显示，过表达OsBIRH1的转基因植物显示出对铜斑癣菌和丁香假单胞菌pv的增强的抗病性。番茄DC3000。同时，与防御相关的基因，例如PR-1，PR-2，PR-5和PDF1.2，在转基因植物中显示出上调的表达。此外，OsBIRH1转基因拟南芥植物还显示出对氧化应激的耐受性增强和氧化防御基因AtApx1，AtApx2和AtFSD1的表达水平升高。结果表明，OsBIRH1编码功能性DEAD-box RNA解旋酶，并在针对生物和非生物胁迫的防御反应中发挥重要作用。

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