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首页> 外文期刊>Journal of Experimental Botany >Identification of woolliness response genes in peach fruit after post-harvest treatments
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Identification of woolliness response genes in peach fruit after post-harvest treatments

机译：桃果实采后处理后毛羽反应基因的鉴定

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Woolliness is a physiological disorder of peaches and nectarines that becomes apparent when fruit are ripened after prolonged periods of cold storage. This disorder is of commercial importance since shipping of peaches to distant markets and storage before selling require low temperature. However, knowledge about the molecular basis of peach woolliness is still incomplete. To address this issue, a nylon macroarray containing 847 non-redundant expressed sequence tags (ESTs) from a ripe peach fruit cDNA library was developed and used. Gene expression changes of peach fruit (Prunus persica cv. O'Henry) ripened for 7 d at 21 °C (juicy fruit) were compared with those of fruit stored for 15 d at 4 °C and then ripened for 7 d at 21 °C (woolly fruit). A total of 106 genes were found to be differentially expressed between juicy and woolly fruit. Data analysis indicated that the activity of most of these genes (90%) was repressed in the woolly fruit. In cold-stored peaches (cv. O'Henry), the expression level of selected genes (cobra, endopolygalacturonase, cinnamoyl-CoA-reductase, and rab11) was lower than in the juicy fruit, and it remained low in woolly peaches after ripening, a pattern that was conserved in woolly fruit from two other commercial cultivars (cv. Flamekist and cv. Elegant Lady). In addition, the results of this study indicate that molecular changes during fruit woolliness involve changes in the expression of genes associated with cell wall metabolism and endomembrane trafficking. Overall, the results reported here provide an initial characterization of the transcriptome activity of peach fruit under different post-harvest treatments.
机译：毛泡现象是桃子和油桃的一种生理失调症，在长时间冷藏后果实成熟后会变得很明显。由于将桃子运送到遥远的市场并在销售前进行存储需要低温，因此这种疾病具有商业重要性。但是，关于桃子羊毛质分子基础的知识仍然不完整。为了解决这个问题，开发并使用了包含来自成熟桃果实cDNA文库的847个非冗余表达序列标签（EST）的尼龙宏阵列。比较在21°C熟化7天（多汁的水果）的桃果实（多汁的李）的基因表达变化与在4°C储存15 d然后在21°熟化7 d的水果的基因表达变化C（毛茸茸的水果）。发现共有106个基因在多汁的和羊毛的水果之间差异表达。数据分析表明，大多数基因（> 90％）的活性在羊毛果中被抑制。在冷藏桃子中（c。O'Henry），所选基因（眼镜蛇，内聚半乳糖醛酸酶，肉桂酰辅酶A-还原酶和rab11）的表达水平低于多汁水果，成熟后在桃子中仍然较低，这种模式在其他两个商业栽培品种（cv。Flamekist和cv。Elegant Lady）的羊毛果实中得以保存。此外，这项研究的结果表明，在果实加粗过程中的分子变化涉及与细胞壁代谢和内膜运输相关的基因表达的变化。总的来说，这里报道的结果提供了桃果实在不同收获后处理下转录组活性的初步表征。

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