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首页> 外文期刊>Journal of Experimental Botany >The organ-dependent abundance of a Solanum lipid transfer protein is up-regulated upon osmotic constraints and associated with cold acclimation ability
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The organ-dependent abundance of a Solanum lipid transfer protein is up-regulated upon osmotic constraints and associated with cold acclimation ability

机译：茄脂脂质转移蛋白的器官依赖性丰度在渗透约束下上调，并与冷驯化能力有关

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摘要

The expression of a gene isolated from cDNA differential screening and encoding a lipid transfer protein, designated as SsLTP1, was analysed at the protein level in two groups of Solanum species and lines differing in cold acclimation capacity. Under control conditions, the SsLTP1 was localized in all aerial organs of S. sogarandinum and S. tuberosum plants. Western analysis of subcellular extracts indicated that the protein possesses an intracellular localization. The protein abundance was found to vary as a function of organ type, the highest levels being observed in flowers, stems, and young leaves. During low temperature treatment, no change in protein level was noticed in either the S. tuberosum cv. Irga, which displays a low capacity for cold acclimation, or in a S. sogarandinum line which has lost its cold acclimation capacity. By contrast, low temperature induced a noticeable increase in SsLTP1 level in stems and leaves of S. sogarandinum and S. tuberosum cv. Ursus plants, which are able to acclimate to cold, indicating that SsLTP1 could participate in the processes leading to freezing tolerance. In other respects, SsLTP1 accumulation was observed both in cold-acclimating and in non-acclimating Solanum species when subjected to water deficit or to salt treatment. These data indicate that SsLTP1 gene expression is regulated in an organ-dependent manner and through distinct pathways under non-freezing low temperature and during osmotic treatments.
机译：在两组茄属和冷适应能力不同的品系的蛋白质水平上，分析了从cDNA差异筛选中分离并编码称为SsLTP1的脂质转移蛋白的基因的表达。在控制条件下，SsLTP1定位在茄子和马铃薯的所有气生器官中。对亚细胞提取物的Western分析表明该蛋白具有细胞内定位。发现蛋白质丰度随器官类型而变化，在花，茎和幼叶中观察到最高水平。在低温处理过程中，马铃薯中的任何一种都没有发现蛋白质水平的变化。 Irga的冷适应能力低，或者在S. sogarandinum品系中失去了冷适应能力。相比之下，低温诱导S. sogarandinum和S. tuberosum cv茎和叶中SsLTP1水平显着增加。能够适应寒冷的熊类植物，表明SsLTP1可能参与导致耐寒性的过程。在其他方面，当进行缺水或盐处理时，在冷适应和非适应性茄属植物中均观察到SsLTP1的积累。这些数据表明，SsLTP1基因表达以器官依赖的方式受到调节，并且在非冷冻低温和渗透处理过程中通过不同的途径被调控。

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    《Journal of Experimental Botany》 |2008年第8期|p.2191-2203|共13页
  • 作者

    Agnieszka Kiełbowicz-Matuk1 Pascal Rey2 and Tadeusz Rorat1*;

  • 作者单位

    1Institute of Plant Genetics, Polish Academy of Sciences, Strzeszyńska 34, 60-479 Poznań, Poland 2 CEA, IBEB, SBVME, LEMP, Laboratoire d'Ecophysiologie Moléculaire des Plantes, UMR 6191 CNRS-CEA-Université de la Méditerranée, 13108 Saint-Paul-lez-Durance, Cedex, France;

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