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首页> 外文期刊>Journal of Experimental Botany >Is internucleosomal DNA fragmentation an indicator of programmed death in plant cells?
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Is internucleosomal DNA fragmentation an indicator of programmed death in plant cells?

机译：核小体间DNA断裂是否指示植物细胞中程序性死亡？

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摘要

Specific DNA fragmentation into oligonucleosomal units occurs during programmed cell death (PCD) in both animal and plant cells, usually being regarded as an indicator of its apoptotic character. This internucleosomal DNA fragmentation is demonstrated in tobacco suspension and leaf cells, which were killed immediately by freezing in liquid nitrogen, and homogenization or treatment with Triton X-100. Although these cells could not activate and realize the respective enzymatic processes in a programmed manner, the character of DNA fragmentation was similar to that in the cells undergoing typical gradual PCD induced by 50 μM CdSO4. This internucleosomal DNA fragmentation was connected with the action of cysteine proteases and the loss of membrane, in particular tonoplast, integrity. The mechanisms of DNase activation in the rapidly killed cells, hypothetical biological relevance, and implications for the classification of cell death are discussed.
机译：在动物和植物细胞的程序性细胞死亡（PCD）期间，发生特定的DNA片段化为寡核小体单位，通常被视为其凋亡特征的指标。这种核糖体间DNA片段化在烟草悬浮液和叶细胞中得到证实，它们通过在液氮中冷冻，匀浆或用Triton X-100处理而立即被杀死。尽管这些细胞不能以编程的方式激活并实现各自的酶促过程，但DNA片段化的特征与经历由50μMCdSO 4 诱导的典型的逐步PCD的细胞相似。这种核小体间的DNA断裂与半胱氨酸蛋白酶的作用和膜的丧失，特别是液泡膜完整性的丧失有关。讨论了DNase在快速杀死的细胞中激活的机制，假设的生物学相关性以及对细胞死亡分类的影响。

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