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首页> 外文期刊>Journal of Experimental Botany >Release of sunflower seed dormancy by cyanide: cross-talk with ethylene signalling pathway
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Release of sunflower seed dormancy by cyanide: cross-talk with ethylene signalling pathway

机译：氰化物释放向日葵种子休眠：与乙烯信号通路的串扰

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Freshly harvested sunflower (Helianthus annuus L.) seeds are considered to be dormant because they fail to germinate at relatively low temperatures (10 °C). This dormancy results mainly from an embryo dormancy and disappears during dry storage. Although endogenous ethylene is known to be involved in sunflower seed alleviation of dormancy, little attention had been paid to the possible role of cyanide, which is produced by the conversion of 1-aminocyclopropane 1-carboxylic acid to ethylene, in this process. The aims of this work were to investigate whether exogenous cyanide could improve the germination of dormant sunflower seeds and to elucidate its putative mechanisms of action. Naked dormant seeds became able to germinate at 10 °C when they were incubated in the presence of 1 mM gaseous cyanide. Other respiratory inhibitors showed that this effect did not result from an activation of the pentose phosphate pathway or the cyanide-insensitive pathway. Cyanide stimulated germination of dormant seeds in the presence of inhibitors of ethylene biosynthesis, but its improving effect required functional ethylene receptors. It did not significantly affect ethylene production and the expression of genes involved in ethylene biosynthesis or in the first steps of ethylene signalling pathway. However, the expression of the transcription factor Ethylene Response Factor 1 (ERF1) was markedly stimulated in the presence of gaseous cyanide. It is proposed that the mode of action of cyanide in sunflower seed dormancy alleviation does not involve ethylene production and that ERF1 is a common component of the ethylene and cyanide signalling pathways.
机译：新鲜收获的向日葵种子（Helianthus annuus L.）被认为处于休眠状态，因为它们无法在较低的温度（10°C）下发芽。这种休眠主要是由胚胎休眠引起的，并且在干燥存储过程中消失。尽管已知内源性乙烯参与向日葵种子休眠的缓解，但是在此过程中，人们很少关注氰化物的可能作用，氰化物是通过将1-氨基环丙烷1-羧酸转化为乙烯而产生的。这项工作的目的是调查外源氰化物是否可以改善休眠向日葵种子的萌发并阐明其推测的作用机理。当裸休眠种子在1 mM气态氰化物存在下孵育时，它们可以在10°C下发芽。其他呼吸抑制剂表明，这种作用不是由磷酸戊糖途径或对氰化物不敏感的途径激活引起的。在乙烯生物合成抑制剂的存在下，氰化物刺激了休眠种子的萌发，但其改善作用需要功能性乙烯受体。它没有显着影响乙烯的生产和参与乙烯生物合成或乙烯信号传导途径第一步的基因表达。但是，在气态氰化物存在下，转录因子乙烯反应因子1（ERF1）的表达受到明显刺激。有人提出，氰化物在减轻向日葵种子休眠中的作用方式不涉及乙烯的产生，而ERF1是乙烯和氰化物信号传导途径的共同组成部分。

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