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首页> 外文期刊>Journal of Experimental Botany >Expression of the β-oxidation gene 3-ketoacyl-CoA thiolase 2 (KAT2) is required for the timely onset of natural and dark-induced leaf senescence in Arabidopsis
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Expression of the β-oxidation gene 3-ketoacyl-CoA thiolase 2 (KAT2) is required for the timely onset of natural and dark-induced leaf senescence in Arabidopsis

机译：需要表达β-氧化基因3-酮酰基-CoA硫解酶2（KAT2），以便及时启动拟南芥自然和黑暗诱导的叶片衰老

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摘要

The onset of leaf senescence is regulated by a complex mechanism involving positive and negative regulators. Among positive regulators, jasmonic acid (JA) accumulates in senescing leaves and the JA-insensitive coi1-1 mutant displays delayed leaf senescence in Arabidopsis. A strong activated expression of the gene coding for the JA-biosynthetic β-oxidation enzyme 3-ketoacyl-CoA thiolase 2 (KAT2) in natural and dark-induced senescing leaves of Arabidopsis thaliana is reported here. By using KAT2::GUS and KAT2::LUC transgenic plants, it was observed that dark-induced KAT2 activation occurred both in excised leaves as well as in whole darkened plants. The KAT2 activation associated with dark-induced senescence occurred soon after a move to darkness, and it preceded the detection of symptoms and the expression of senescence-associated gene (SAG) markers. Transgenic plants with reduced expression of the KAT2 gene showed a significant delayed senescence both in natural and dark-induced processes. The rapid induction of the KAT2 gene in senescence-promoting conditions as well as the delayed senescence phenotype and the reduced SAG expression in KAT2 antisense transgenic plants, point to KAT2 as an essential component for the timely onset of leaf senescence in Arabidopsis.
机译：叶片衰老的发生是由涉及正负调节剂的复杂机制调节的。在正调节剂中，茉莉酸（JA）积累在衰老的叶片中，而JA不敏感的coi1-1突变体在拟南芥中显示出延迟的叶片衰老。此处报道了在拟南芥的天然和黑暗诱导的衰老叶片中编码JA-生物合成β-氧化酶3-酮酰基-CoA硫解酶2（KAT2）的基因的强激活表达。通过使用KAT2 :: GUS和KAT2 :: LUC转基因植物，观察到在被切除的叶片以及整个深色植物中都发生了黑暗诱导的KAT2活化。与黑暗诱导的衰老相关的KAT2激活在转移到黑暗后不久发生，并且先于症状的检测和衰老相关基因（SAG）标记的表达。 KAT2基因表达降低的转基因植物在自然和黑暗诱导的过程中均显示出明显的衰老延迟。在促进衰老的条件下快速诱导KAT2基因，以及在KAT2反义转基因植物中延缓衰老表型和降低SAG表达，都表明KAT2是拟南芥叶片衰老及时发生的重要组成部分。

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  • 来源
    《Journal of Experimental Botany》 |2008年第8期|p.2171-2179|共9页
  • 作者

    Mari Cruz Castillo and José León*;

  • 作者单位

    Instituto de Biología Molecular y Celular de Plantas (CSIC-UPV), Ciudad Politécnica de la Innovación, Edificio 8E, Avenida del Ingeniero Fausto Elio s, E-46022 Valencia, Spain;

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