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首页> 外文期刊>Journal of Experimental Botany >IAA stimulates pollen tube growth and mediates the modification of its wall composition and structure in Torenia fournieri
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IAA stimulates pollen tube growth and mediates the modification of its wall composition and structure in Torenia fournieri

机译：IAA刺激花粉管中花粉管的生长并介导其壁组成和结构的改变

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摘要

The effects of several hormones on pollen tube growth were compared in Torenia fournieri and it was found that IAA was the most effective, stimulating pollen tube growth and causing the shank part of pollen tubes to be slender and straighter. The role of IAA was investigated by studying the changes in ultrastructure and PM H+-ATPase distribution in the pollen tubes and the modification of the tube wall. Using the fluorescent marker FM4-64, together with transmission electron microscopy, it was shown that secretory vesicles and mitochondria increased in IAA-treated tubes. Immunolocalization and fluorescence labelling, together with Fourier-transform infrared analysis, detected that IAA enhanced the level of PM H+-ATPase and the synthesis of pectins, and reduced the cellulose density in pollen tubes. Importantly, to observe the orientation of cellulose microfibrils in pollen tubes in situ, atomic force microscopy was used to examine the ‘intact’ tube wall. Atomic force microscopy images showed that cellulose microfibrils were parallel to each other in the subapical region of IAA-treated tubes, but disorganized in control tubes. All results provided new insights into the functions of cellulose microfibrils in pollen tube growth and direction, and revealed that the IAA-induced changes of pollen tubes were attributed to the increase in secretory vesicles, mitochondria, and PM H+-ATPase, and the modification of pectin and cellulose microfibrils in the tube wall.
机译：在Torenia fournieri中比较了几种激素对花粉管生长的影响，发现IAA是最有效的，它刺激花粉管生长并使花粉管的小腿部分细长而笔直。通过研究花粉管中超微结构和PM H + -ATPase分布的变化以及管壁的修饰来研究IAA的作用。使用荧光标记FM4-64以及透射电子显微镜，表明在IAA处理过的试管中，分泌性囊泡和线粒体增加。免疫定位和荧光标记，再结合傅里叶变换红外分析，发现IAA增强了PM H + -ATPase的水平和果胶的合成，并降低了花粉管中的纤维素密度。重要的是，要观察花粉管中纤维素微纤维的原位，原子力显微镜用于检查“完整”的管壁。原子力显微镜图像显示，在IAA处理过的试管的根尖下区域，纤维素微纤维彼此平行，但在对照试管中却杂乱无章。所有结果为纤维素微纤维在花粉管生长和方向中的功能提供了新的见解，并揭示了IAA诱导的花粉管变化归因于分泌性囊泡，线粒体和PM H + -ATPase，以及果胶和纤维素微纤维在管壁中的修饰。

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    《Journal of Experimental Botany》 |2008年第9期|p.2529-2543|共15页
  • 作者

    Juan-Zi Wu1 Yi Lin2 Xue-Lian Zhang1 Dai-Wen Pang2 and Jie Zhao1*;

  • 作者单位

    1Key Laboratory of the Ministry of Education for Plant Developmental Biology, College of Life Sciences, Wuhan University, Wuhan, 430072, China 2College of Chemistry and Molecular Sciences, and State Key Laboratory of Virology, Wuhan University, Wuhan 430072, China;

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