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首页> 外文期刊>Journal of Experimental Botany >Differential transcript accumulation in Cicer arietinum L. in response to a chewing insect Helicoverpa armigera and defence regulators correlate with reduced insect performance
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Differential transcript accumulation in Cicer arietinum L. in response to a chewing insect Helicoverpa armigera and defence regulators correlate with reduced insect performance

机译：咀嚼昆虫Helicoverpa armigera和防御调节剂对Alicinum L. Cicer的差异转录物积累与降低的昆虫表现相关

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摘要

Monitoring transcriptional reorganization triggered in response to a particular stress is an essential first step for the functional analysis of genes involved in the process. To characterize Cicer arietinum L. defence responses against Helicoverpa armigera feeding, transcript patterns elicited by both herbivore and mechanical wounding were profiled and compared, and the application of defence regulators was assessed. A combination of approaches was employed to develop transcript profiles, including suppression subtractive hybridization (SSH), macroarray, northern blot, and cluster analysis. Of the 63 unique genes isolated, 29 genes expressed differentially when Helicoverpa feeding and wounding responses were compared. Comparative macroarray analyses revealed that most of the Helicoverpa-induced transcripts were methyl jasmonate (MeJA) and ethylene (ET) regulated. The effects of mild insect infestation and the exogenous application of signalling compounds on larval feeding behaviour were also monitored. Bioassays were performed to measure dispersal percentage and growth of larvae on elicited plants. Larvae released on elicited plants had decreased larval performance, demonstrating the central role of induced plant defence against herbivory. Similarly, wounding and exogenous application of MeJA and ET also affected larval growth and feeding behaviour. Our results demonstrated that Helicoverpa attack up-regulated large transcriptional changes and induced chickpea defence responses. Therefore, the results of this study advance the understanding of non-model plant–insect interactions on a broader scale.
机译：监测响应特定压力而触发的转录重组，是对该过程中涉及的基因进行功能分析的重要第一步。为了表征Cicer arietinum L.对棉铃虫取食的防御反应，分析并比较了草食动物和机械性损伤引起的转录本模式，并评估了防御调节剂的应用。采用了多种方法来开发转录本图谱，包括抑制消减杂交（SSH），大阵列，RNA印迹和聚类分析。在分离的63个独特基因中，当比较Helicoverpa的进食和受伤反应时，有29个基因差异表达。比较性宏阵列分析显示，Helicoverpa诱导的大多数转录本均由茉莉酸甲酯（MeJA）和乙烯（ET）调节。还监测了轻度昆虫侵染的影响和外源施用信号化合物对幼虫摄食行为的影响。进行生物测定以测量幼虫在引诱植物上的分散百分比和生长。在诱发的植物上释放的幼虫具有降低的幼虫性能，证明了诱导的植物对草食动物防御的核心作用。同样，MeJA和ET的创伤和外源施用也影响幼虫的生长和摄食行为。我们的结果表明，Helicoverpa攻击上调大的转录变化并诱导鹰嘴豆防御反应。因此，这项研究的结果在更广泛的程度上提高了对非模型植物与昆虫相互作用的理解。

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