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首页> 外文期刊>Journal of Experimental Botany >Localization of sucrose synthase in developing seed and siliques of Arabidopsis thaliana reveals diverse roles for SUS during development
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Localization of sucrose synthase in developing seed and siliques of Arabidopsis thaliana reveals diverse roles for SUS during development

机译：蔗糖合酶在拟南芥种子和长角果中的定位揭示了发育过程中SUS的不同作用

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摘要

This study investigated the roles of sucrose synthase (SUS) in developing seeds and siliques of Arabidopsis thaliana. Enzyme activity assays showed that SUS activity was highest in developing whole siliques and young rosette leaves compared with other tissues including mature leaves, stems, and flowers. Surprisingly, quantitative PCR analyses revealed little correlation between SUS activity and transcript expression, which indicated the importance of examining the role of SUS at the protein level. Therefore, immunolocalization was performed over a developmental time course to determine the previously unreported cellular localization of SUS in Arabidopsis seed and silique tissues. At 3 d and 10 d after flowering (daf), SUS protein localized to the silique wall, seed coat, funiculus, and endosperm. By 13 daf, SUS protein was detected in the embryo and aleurone layer, but was absent from the seed coat and funiculus. Starch grains were also present in the seed coat at 3 and 10 daf, but were absent at 13 daf. Co-localization of SUS protein and starch grains in the seed coat at 3 and 10 daf indicates that SUS may be involved in temporary starch deposition during the early stages of seed development, whilst in the later stages SUS metabolizes sucrose in the embryo and cotyledon. Within the silique wall, SUS localized specifically to the companion cells, indicating that SUS activity may be required to provide energy for phloem transport activities in the silique wall. The results highlight the diverse roles that SUS may play during the development of silique and seed in Arabidopsis.
机译：这项研究调查了蔗糖合酶（SUS）在拟南芥种子和长角果发育中的作用。酶活性测定表明，与其他组织（包括成熟叶，茎和花）相比，在整个长角果和年轻莲座叶中，SUS活性最高。出乎意料的是，定量PCR分析显示SUS活性与转录本表达之间几乎没有相关性，这表明了检查SUS在蛋白质水平上的作用的重要性。因此，在发育过程中进行了免疫定位，以确定在拟南芥种子和长角果组织中SUS以前未报道的细胞定位。开花后3天和10天（daf），SUS蛋白定位在角果皮壁，种皮，真菌和胚乳上。到13 daf时，在胚胎和糊粉层中检测到SUS蛋白，但种皮和真菌中却没有SUS蛋白。种皮中还存在3和10 daf的淀粉粒，但13 daf则不存在。 SUS蛋白和淀粉颗粒在3 daf和10 daf处的共定位表明SUS可能在种子发育的早期阶段参与了暂时的淀粉沉积，而在后期阶段SUS则在胚芽和子叶中代谢蔗糖。在长角果壁内，SUS专门定位于陪伴细胞，这表明可能需要SUS活性才能为长角果壁中的韧皮部运输活动提供能量。结果突出了SUS在拟南芥的长角果和种子发育过程中可能发挥的不同作用。

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