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首页> 外文期刊>Journal of Experimental Botany >An isoform of Arabidopsis myosin XI interacts with small GTPases in its C-terminal tail region
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An isoform of Arabidopsis myosin XI interacts with small GTPases in its C-terminal tail region

机译：拟南芥肌球蛋白XI的同工型在其C末端尾部区域与小GTPase相互作用。

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摘要

Myosin XI, a class of myosins expressed in plants is believed to be responsible for cytoplasmic streaming and the translocation of organelles and vesicles. To gain further insight into the translocation of organelles and vesicles by myosin XI, an isoform of Arabidopsis myosin XI, MYA2, was chosen and its role in peroxisome targeting was examined. Using the yeast two-hybrid screening method, two small GTPases, AtRabD1 and AtRabC2a, were identified as factors that interact with the C-terminal tail region of MYA2. Both recombinant AtRabs tagged with His bound to the recombinant C-terminal tail region of MYA2 tagged with GST in a GTP-dependent manner. Furthermore, AtRabC2a was localized on peroxisomes, when its CFP-tagged form was expressed transiently in protoplasts prepared from Arabidopsis leaf tissue. It is suggested that MYA2 targets the peroxisome through an interaction with AtRabC2a.
机译：据信，肌球蛋白XI是植物中表达的一类肌球蛋白，它负责细胞质流以及细胞器和囊泡的转运。为了进一步了解肌球蛋白XI迁移细胞器和囊泡，选择了拟南芥肌球蛋白XI的同工型MYA2，并检查了其在过氧化物酶体靶向中的作用。使用酵母双杂交筛选方法，两个小的GTPases AtRabD1和AtRabC2a被确定为与MYA2 C末端尾部区域相互作用的因子。标记有His的两个重组AtRabs均以依赖GTP的方式与标记有GST的MYA2的重组C末端尾部区域结合。此外，当AtRabC2a的CFP标签形式在由拟南芥叶组织制备的原生质体中瞬时表达时，它位于过氧化物酶体上。建议MYA2通过与AtRabC2a相互作用靶向过氧化物酶体。

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