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首页> 外文期刊>Journal of Experimental Botany >Identification of target genes for a MYB-type anthocyanin regulator in Gerbera hybrida
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Identification of target genes for a MYB-type anthocyanin regulator in Gerbera hybrida

机译：非洲菊MYB型花色苷调节子的目标基因的鉴定

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Genetic modification of the flavonoid pathway has been used to produce novel colours and colour patterns in ornamental plants as well as to modify the nutritional and pharmaceutical properties of food crops. It has been suggested that co-ordinate control of multiple steps of the pathway with the help of regulatory genes would lead to a more predictable control of metabolic flux. Regulation of anthocyanin biosynthesis has been studied in a common ornamental plant, Gerbera hybrida (Asteraceae). An R2R3-type MYB factor, GMYB10, shares high sequence similarity and is phylogenetically grouped together with previously characterized regulators of anthocyanin pigmentation. Ectopic expression of GMYB10 leads to strongly enhanced accumulation of anthocyanin pigments as well as to an altered pigmentation pattern in transgenic gerbera plants. Anthocyanin analysis indicates that GMYB10 specifically induces cyanidin biosynthesis in undifferentiated callus and in vegetative tissues. Furthermore, in floral tissues enhanced pelargonidin production is detected. Microarray analysis using the gerbera 9K cDNA array revealed a highly predicted set of putative target genes for GMYB10 including new gene family members of both early and late biosynthetic genes of the flavonoid pathway. However, completely new candidate targets, such as a serine carboxypeptidase-like gene as well, as two new MYB domain factors, GMYB11 and GMYB12, whose exact function in phenylpropanoid biosynthesis is not clear yet, were also identified.
机译：类黄酮途径的遗传修饰已用于在观赏植物中产生新颖的颜色和颜色图案，以及修饰食用作物的营养和药物特性。已经提出，借助于调节基因来协调途径的多个步骤的控制将导致对代谢通量的更可预测的控制。在常见的观赏植物非洲菊（菊科）中已研究了花色苷生物合成的调控。 R2R3型MYB因子GMYB10具有高度的序列相似性，并与以前鉴定的花色苷色素沉着调节剂一起进行系统发育分组。 GMYB10的异位表达可大大增强花色苷色素的积累，并在转基因非洲菊植株中引起色素沉着模式的改变。花青素分析表明，GMYB10在未分化的愈伤组织和营养组织中特异性诱导花青素的生物合成。此外，在花卉组织中检测到增强的pelargonidin产生。使用非洲菊9K cDNA阵列进行的微阵列分析显示，高度预测的GMYB10假定靶基因组包括黄酮途径的早期和后期生物合成基因的新基因家族成员。但是，还确定了全新的候选靶标，例如丝氨酸羧肽酶样基因，以及两个新的MYB结构域因子GMYB11和GMYB12，它们在苯丙烷类生物合成中的确切功能尚不清楚。

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