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首页> 外文期刊>Journal of Experimental Botany >Expression of carotenoid biosynthesis genes during carrot root development
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Expression of carotenoid biosynthesis genes during carrot root development

机译：胡萝卜根发育过程中类胡萝卜素生物合成基因的表达

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摘要

Carotenogenesis has been extensively studied in fruits and flower petals. Transcriptional regulation is thought to be the major factor in carotenoid accumulation in these organs. However, little is known about regulation in root organs. The root carotenoid content of carrot germplasm varies widely. The present study was conducted to investigate transcriptional regulation of carotenoid biosynthesis genes in relation to carotenoid accumulation during early carrot root development and up to 3 months after sowing. HPLC carotenoid content analysis and quantitative RT-PCR were compared to quantify the expression of eight genes encoding carotenoid biosynthesis enzymes during the development of white, yellow, orange, and red carrot roots. The genes chosen encode phytoene synthase (PSY1 and PSY2), phytoene desaturase (PDS), ζ-carotene desaturase (ZDS1 and ZDS2), lycopene ε-cyclase (LCYE), lycopene β-cyclase (LCYB1), and zeaxanthin epoxidase (ZEP). All eight genes were expressed in the white cultivar even though it did not contain carotenoids. By contrast with fruit maturation, the expression of carotenogenic genes began during the early stages of development and then progressively increased for most of these genes during root development as the total carotenoid level increased in coloured carrots. The high expression of genes encoding LCYE and ZDS noted in yellow and red cultivars, respectively, might be consistent with the accumulation of lutein and lycopene, respectively. The results showed that the accumulation of total carotenoids during development and the accumulation of major carotenoids in the red and yellow cultivars might partially be explained by the transcriptional level of genes directing the carotenoid biosynthesis pathway.
机译：在水果和花瓣中对胡萝卜素的形成进行了广泛的研究。转录调节被认为是这些器官中类胡萝卜素积累的主要因素。但是，对根器官的调节知之甚少。胡萝卜种质的根类胡萝卜素含量差异很大。本研究的目的是调查胡萝卜胡萝卜根发育早期和播种后3个月内与类胡萝卜素积累相关的类胡萝卜素生物合成基因的转录调控。比较了HPLC类胡萝卜素含量分析和定量RT-PCR，以定量在白色，黄色，橙色和红色胡萝卜根发育过程中编码类胡萝卜素生物合成酶的八个基因的表达。选择的基因编码八氢番茄红素合酶（PSY1和PSY2），八氢番茄红素去饱和酶（PDS），ζ-胡萝卜素脱氢酶（ZDS1和ZDS2），番茄红素ε-环化酶（LCYE），番茄红素β-环化酶（LCYB1）和玉米黄质环氧化酶（ZEP） 。所有八个基因均在白色品种中表达，即使它不包含类胡萝卜素。与水果成熟相反，类胡萝卜素基因的表达在发育的早期阶段开始，然后在根部发育过程中，大多数这些基因的表达逐渐增加，因为有色胡萝卜中总类胡萝卜素水平增加。分别在黄色和红色品种中注意到的编码LCYE和ZDS的基因的高表达可能分别与叶黄素和番茄红素的积累相一致。结果表明，红色和黄色品种在发育过程中总类胡萝卜素的积累和主要类胡萝卜素的积累可能部分由指导类胡萝卜素生物合成途径的基因的转录水平来解释。

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