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首页> 外文期刊>Journal of Experimental Botany >Factors involved in root formation in Medicago truncatula
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Factors involved in root formation in Medicago truncatula

机译：紫花苜蓿根系形成的相关因素

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The fact that auxin induces root formation has been known for more than half a century. However, despite the recent progress in this field, neither the molecular processes in which the auxin-responsive genes leading to root formation nor the interactions between phytohormones and other bioactive molecules during the commitment phase of root formation are well understood. Here the effect of biomolecules such as cytokinin, glutathione, and flavonoids, as well as the expression of several transcription factors in in vitro root formation in model legume Medicago truncatula are presented. It was demonstrated that auxin NAA (1-naphthaleneacetic acid) pretreatment for 7 d can irreversibly interrupt somatic embryo formation, whilst both reduced and oxidized forms of glutathione enhance root formation via a mechanism independent of ethylene perception, as determined by analysis of the ethylene-insensitive skl mutant. It was also shown that quercetin and the well-known auxin transport inhibitor NPA (N-1-naphthylphthalamic acid), which has a similar structure to quercetin, and isoflavonoids formononetin and genistein caused severe reduction in root formation. Also, the relative expression of several transcription factors was analysed in 1-week-old NAA-treated explants (stem cell niche formation stage), in NAA- and BAP-treated explants (no root formation), and in the roots of germinated seeds. The results showed, for the first time in a legume, that the transcription factors homeodomain WOX5 and the AP2-domain containing PLETHORA1 and 2, BABY BOOM1 were strongly induced by auxin addition, while cytokinin addition dramatically reduced their expression, indicating a role for these genes in the formation of root stem cell niches.
机译：生长素诱导根形成的事实已经有半个多世纪了。然而，尽管该领域最近取得了进展，但是尚不清楚植物生长素应答基因导致根形成的分子过程或植物激素与其他生物活性分子在根形成定型阶段之间的相互作用。这里介绍了生物分子如细胞分裂素，谷胱甘肽和类黄酮的作用，以及几种在豆科植物紫花苜蓿模型中形成根的转录因子的表达。已证明生长素NAA（1-萘乙酸）预处理7 d可以不可逆地中断体细胞胚的形成，而还原型和氧化型谷胱甘肽均通过独立于乙烯感知的机理来增强根形成，这通过乙烯-乙烯分析确定不敏感的skl突变体。还显示出槲皮素和众所周知的生长素转运抑制剂NPA（N-1-萘基邻苯二甲酸酯）具有与槲皮素相似的结构，异黄酮类formononetin和染料木黄酮会导致根系形成的严重减少。同样，在1周龄NAA处理的外植体（干细胞生态位形成阶段），NAA和BAP处理的外植体（无根形成）以及发芽种子的根中分析了几种转录因子的相对表达。 。结果首次在豆科植物中发现，生长素添加强烈诱导了转录因子同源结构域WOX5和包含PLETHORA1和2的AP2结构域，BABY BOOM1被强烈诱导，而细胞分裂素的添加显着降低了它们的表达，表明了它们的作用。基因在根干细胞生态位的形成。

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  • 来源
    《Journal of Experimental Botany》 |2007年第3期|439-451|共13页
  • 作者

    Nijat Imin Mahira Nizamidin Tina Wu and Barry G. Rolfe;

  • 作者单位

    Australian Research Council Centre of Excellence for Integrative Legume Research Genomic Interactions Group Research School of Biological Sciences Australian National University Canberra City ACT 2601 Australia;

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