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首页> 外文期刊>Journal of Experimental Botany >Expression of polygalacturonases and evidence to support their role during cell separation processes in Arabidopsis thaliana
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Expression of polygalacturonases and evidence to support their role during cell separation processes in Arabidopsis thaliana

机译：多聚半乳糖醛酸酶的表达及其在拟南芥细胞分离过程中支持其作用的证据

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摘要

Polygalacturonases (PGs) have been proposed to play an important role in the process of cell separation. The Arabidopsis thaliana genome contains 69 annotated genes that by amino acid homology and transcript organization could be classified as putative PGs and these can be grouped into multiple clades. An analysis of five members located in two separate clades, using reporter fusion constructs and reverse transcription-PCR, revealed that whilst these PGs exhibit high sequence similarity they have distinct patterns of spatial and temporal expression. Sites of expression include the aleurone and endosperm cells surrounding the emerging radicle in a germinating seed, the cortical cells adjacent to the developing lateral root, the abscission zones of floral organs, the dehiscence zone of anthers and siliques, and pollen grains. Silencing of an abscission-related PG (At2g41850), using a T-DNA insertion strategy, delayed the time-course of floral organ loss but did not prevent shedding from taking place. These observations are discussed with regard to the contribution that PGs may play during the life cycle of a plant.
机译：已经提出，聚半乳糖醛酸酶（PGs）在细胞分离过程中起重要作用。拟南芥基因组包含69个带注释的基因，根据氨基酸同源性和转录本组织，这些基因可以归类为推定的PG，并且可以分为多个进化枝。使用记者融合构建体和逆转录PCR对位于两个不同进化枝中的五个成员进行的分析显示，尽管这些PG表现出高度的序列相似性，但它们具有不同的时空表达模式。表达部位包括发芽种子中新兴胚根周围的糊粉和胚乳细胞，与发育中的侧根相邻的皮质细胞，花器官的脱落区，花药和长角果的开裂区以及花粉粒。使用T-DNA插入策略沉默与脱落相关的PG（At2g41850），可以延迟花器官损失的时间，但不能阻止脱落的发生。讨论了PG在植物生命周期中可能发挥的作用。

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