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首页> 外文期刊>Journal of Experimental Botany >Identification of dominant mutations that confer increased aluminium tolerance through mutagenesis of the Al-sensitive Arabidopsis mutant, als3-1
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Identification of dominant mutations that confer increased aluminium tolerance through mutagenesis of the Al-sensitive Arabidopsis mutant, als3-1

机译：通过诱变铝敏感拟南芥突变体ALS3-1鉴定赋予铝耐受性的显性突变

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Aluminium (Al) toxicity is a global agricultural problem that occurs in acid soil environments and severely limits root growth and crop productivity. The isolation and characterization of a gene, ALS3, which is absolutely required by Arabidopsis seedlings for growth in an Al-toxic environment was reported previously. Since the als3-1 loss-of-function mutant has extreme root growth inhibition even in the presence of very low levels of Al, it was an excellent candidate for using a mutagenesis approach to identify suppressor mutations that would increase either Al resistance or tolerance in Arabidopsis roots. EMS-mutagenized als3-1 seedlings were screened for mutants that could sustain root growth in an Al-containing environment that is highly toxic to als3-1 but not Col-0 wt. This approach resulted in identification of 12 strong suppressor mutants that reversed the als3-1 phenotype and grew as well or better than Col-0 wt in the presence of high levels of Al. Subsequent analysis of three representative suppressor mutants revealed that the phenotype of each probably arises from dominant gain-of-function mutations at the same locus. Detailed analysis of one of these, alt1-1 (Altolerant), suggests that this mutation positively impacts Al resistance in a manner dependent on pH adjustment rather than enhanced Al exclusion. Identification of these suppressor mutations, should not only further elucidate the biochemical and molecular mechanisms underlying Al toxicity and tolerance but also will develop a collection of mutations that may be useful for engineering crop plants that can grow and thrive in Al-toxic environments.
机译：铝（Al）毒性是一个全球性农业问题，发生在酸性土壤环境中，严重限制了根系生长和作物生产力。先前已经报道了拟南芥幼苗在铝毒性环境中生长所绝对需要的基因ALS3的分离和鉴定。由于als3-1功能丧失型突变体即使在极低水平的Al存在下也具有极高的根系生长抑制作用，因此它是使用诱变方法鉴定抑制突变的极佳候选者，该突变会增加Al的抗性或耐受性拟南芥根。筛选了EMS诱变的ALS3-1幼苗中可以维持在对ALS3-1毒性高但对Col-0重量无毒的含铝环境中根系生长的突变体。这种方法导致鉴定了12个强抑制突变体，这些突变体逆转了als3-1表型，并且在高水平的Al存在下与Col-0 wt一样好或更好地生长。随后对三个代表性抑制突变体的分析表明，每个突变体的表型可能来自同一基因座上的显性功能获得性突变。对其中之一alt1-1（耐盐）的详细分析表明，该突变以依赖于pH调节的方式（而不是增强的铝排阻）对Al的抗性产生积极影响。这些抑制突变的鉴定，不仅应进一步阐明铝毒性和耐受性的生化和分子机制，而且还将开发出一系列突变，这些突变可能对工程化的植物植物有用，这些植物可以在铝毒性环境中生长和繁盛。

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