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首页> 外文期刊>Journal of Experimental Botany >A new, non-perturbing, sampling procedure in tracer exchange measurements
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A new, non-perturbing, sampling procedure in tracer exchange measurements

机译：示踪剂交换测量中新的，不受干扰的采样程序

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摘要

An isotope procedure for the tracing of ion fluxes and rate constants in intact plants is presented and applied to 42K-labelled potassium fluxes in cells of intact barley (Hordeum vulgare L.) roots. This procedure differs from conventional tracer efflux protocols in that tracer accrual in the external solution bathing the labelled roots is continually monitored by solution subsampling, whereas conventional protocols involve monitoring the specific-activity decline in a sequence of eluates that wash out tracer released by roots. The new technique minimizes physical disturbance to the plant system, while permitting excellent time resolution of efflux kinetics. In the high-affinity transport (HATS) range, the flux and exchange parameters determined using this method showed close agreement with those found using a conventional protocol. However, in the low-affinity transport (LATS) range, substantially higher influx and efflux were seen than are normally observed with conventional tracer techniques. It is shown that this difference is attributable to the greater disturbance-sensitivity of LATS transport, and conclude that the measurement of fluxes is much more difficult in this transport range than in the disturbance-resistant HATS range.
机译：提出了一种同位素方法，用于追踪完整植物中离子通量和速率常数，并将其应用于完整大麦（Hordeum vulgare L.）根细胞中 42 K标记的钾通量。此过程与常规示踪剂外排规程的不同之处在于，通过溶液二次采样不断监测标记了根的外部溶液中示踪剂的积累，而常规规程涉及监测洗出根的示踪剂的洗脱液的比活度下降。这项新技术最大程度地减少了对植物系统的物理干扰，同时使流出动力学具有出色的时间分辨能力。在高亲和力传输（HATS）范围内，使用此方法确定的通量和交换参数显示与使用常规协议发现的参数紧密一致。但是，在低亲和力转运（LATS）范围内，观察到的流入量和流出量比常规示踪剂技术通常观察到的要高得多。结果表明，这种差异归因于LATS运输的更高的干扰敏感性，并且得出结论，在此运输范围内，通量的测量要比在抗干扰的HATS范围内更加困难。

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