...
  • 主题
高级搜索  >
历史搜索 清空历史
首页> 外文期刊>Journal of Experimental Botany >Physiological and biochemical responses of fruit exocarp of tomato (Lycopersicon esculentum Mill.) mutants to natural photo-oxidative conditions
【24h】

Physiological and biochemical responses of fruit exocarp of tomato (Lycopersicon esculentum Mill.) mutants to natural photo-oxidative conditions

机译：番茄（Lycopersicon esculentum Mill。）果实外果皮对自然光氧化条件的生理生化响应

获取原文
获取原文并翻译 | 示例
           
页面导航
  • 摘要
  • 著录项
  • 相似文献
  • 相关主题

摘要

Photo-oxidative stress was imposed under natural solar radiation on exposed and shaded sections of detached fruit of immature green tomato (Lycopersicon esculentum Miller=Solanum lycopersicum L.) mutants (anthocyanin absent, β-carotene, Delta, and high pigment-1) and their nearly isogenic parents (‘Ailsa Craig’ and ‘Rutgers’). After 5 h exposure to high solar irradiance, either with or without ultraviolet (UV) radiation, surface colour changes, pigment composition, photosynthetic efficiency, antioxidant metabolites and enzyme activities, and selected flavonoids and antioxidant proteins in exocarp tissue were evaluated. The imposed photo-oxidative stress reproduced the symptoms observed on attached fruit. Both high temperature and solar irradiance caused fruit surface discoloration with faster degradation of chlorophyll (Chl) than carotenoids (Car), leading to an increase in the Car/Chl ratio. Surface bleaching was mostly caused by visible light, whereas elevated temperatures were mostly responsible for the inactivation of photosynthesis, measured as decreased Fv/Fm. Ascorbate, glutathione, and total soluble protein concentrations decreased in the exocarp as the duration of exposure increased. Specific activities of superoxide dismutase, ascorbate peroxidase, dehydroascorbate reductase, monodehydroascorbate reductase (MDHAR), glutathione reductase (GR), and catalase increased with exposure, suggesting that these proteins were conserved during the imposed stress. GR protein expression remained stable during the imposed stress, whereas, MDHAR protein expression increased. Quercetin and kaempferol concentrations increased rapidly upon exposure, but not to UV radiation, suggesting rapid photo-protection in response to visible light; however, naringenin synthesis was not induced. The apparent increased tolerance of hp-1 fruit is discussed.
机译：在自然阳光照射下，未成熟的绿色番茄（Lycopersicon esculentum Miller = Solanum lycopersicum L.）突变体（缺少花青素，β-胡萝卜素，δ和高色素-1）的裸露果实和阴影部分施加了光氧化应激。他们几乎是同基因的父母（'Ailsa Craig'和'Rutgers'）。在有或没有紫外线（UV）照射下暴露5 h后，评估了外果皮组织中的类黄酮和抗氧化剂蛋白，无论有无紫外线（UV）辐射，表面颜色变化，色素成分，光合作用效率，抗氧化剂代谢产物和酶活性以及选定的类黄酮和抗氧化剂蛋白质都经过评估。施加的光氧化胁迫重现了附着水果上的症状。高温和太阳辐射都导致果实表面变色，叶绿素（Chl）的降解比类胡萝卜素（Car）快，导致Car / Chl比增加。表面漂白主要由可见光引起，而升高的温度主要是导致光合作用失活的原因，以F v / F m 的降低来衡量。随着暴露时间的延长，外果皮中的抗坏血酸，谷胱甘肽和总可溶性蛋白浓度降低。超氧化物歧化酶，抗坏血酸过氧化物酶，脱氢抗坏血酸还原酶，单脱氢抗坏血酸还原酶（MDHAR），谷胱甘肽还原酶（GR）和过氧化氢酶的比活度随暴露而增加，表明这些蛋白质在施加压力时是保守的。在施加压力期间，GR蛋白表达保持稳定，而MDHAR蛋白表达增加。槲皮素和山emp酚的浓度在暴露后迅速增加，但没有受到紫外线辐射，表明对可见光有快速的光保护作用。然而，没有诱导柚皮苷合成。讨论了hp-1水果明显增加的耐受性。

著录项

相似文献

  • 外文文献
  • 中文文献
  • 专利
获取原文

客服邮箱：kefu@zhangqiaokeyan.com

京公网安备：11010802029741号 ICP备案号：京ICP备15016152号-6 六维联合信息科技 (北京) 有限公司©版权所有

  • 客服微信

  • 服务号