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首页> 外文期刊>Journal of Experimental Botany >Mutant identification and characterization of the laccase gene family in Arabidopsis
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Mutant identification and characterization of the laccase gene family in Arabidopsis

机译：拟南芥漆酶基因家族的突变鉴定与表征

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摘要

Laccases, EC 1.10.3.2 or p-diphenol:dioxygen oxidoreductases, are multi-copper containing glycoproteins. Despite many years of research, genetic evidence for the roles of laccases in plants is mostly lacking. In this study, a reverse genetics approach was taken to identify T-DNA insertional mutants (the SALK collection) available for genes in the Arabidopsis laccase family. Twenty true null mutants were confirmed for 12 laccase genes of the 17 total laccase genes (AtLAC1 to AtLAC17) in the family. By examining the mutants identified, it was found that four mutants, representing mutations in three laccase genes, showed altered phenotypes. Mutants for AtLAC2, lac2, showed compromised root elongation under PEG-induced dehydration conditions; lac8 flowered earlier than wild-type plants, and lac15 showed an altered seed colour. The diverse phenotypes suggest that laccases perform different functions in plants and are not as genetically redundant as previously thought. These mutants will prove to be valuable resources for understanding laccase functions in vivo.
机译：漆酶，EC 1.10.3.2或对二酚：双氧氧化还原酶是含有多铜的糖蛋白。尽管进行了多年的研究，但大多缺乏有关漆酶在植物中作用的遗传证据。在这项研究中，采用反向遗传学方法来鉴定可用于拟南芥漆酶家族基因的T-DNA插入突变体（SALK集合）。确认了家族中17个漆酶基因（AtLAC1至AtLAC17）中的12个漆酶基因的二十个真空突变体。通过检查鉴定出的突变体，发现代表三个漆酶基因突变的四个突变体表现出改变的表型。 AtLAC2 lac2突变体在PEG诱导的脱水条件下显示出受损的根伸长。 lac8比野生型植物更早开花，而lac15显示出改变的种子颜色。不同的表型表明漆酶在植物中执行不同的功能，并且遗传上没有以前想象的那么多。这些突变体将被证明是了解体内漆酶功能的宝贵资源。

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