• 主题
高级搜索  >
历史搜索 清空历史
首页> 外文期刊>Journal of Experimental Marine Biology and Ecology >Species- and stage-specific barnacle larval distributions obtained from AUV sampling and genetic analysis in Buzzards Bay, Massachusetts, USA
【24h】

Species- and stage-specific barnacle larval distributions obtained from AUV sampling and genetic analysis in Buzzards Bay, Massachusetts, USA

机译：从AUV采样和遗传分析获得的物种和阶段特定的藤壶幼虫分布，美国马萨诸塞州的巴扎德斯湾

获取原文
获取原文并翻译 | 示例
           
页面导航
  • 摘要
  • 著录项
  • 相似文献
  • 相关主题

摘要

A new method to autonomously collect larvae and environmental variables such as temperature, salinity, and circulation is described. A large volume in situ pumping system, recently developed for discrete biogeochemical sample collection in deep-sea environments, was adapted and mounted to the autonomous underwater vehicle (AUV) REMUS 600 for autonomous larval and environmental sampling in coastal waters. To assess the performance of this system, the distribution of barnacle larvae was assessed in March 2014 with two transects perpendicular to the coastline (similar to 9.9 and 11.2 km) in Buzzards Bay, Massachusetts, USA. The second transect included a complex sampling mission through a relatively deeper channel, and sampling at discrete depth intervals. In this deployment nearshore and surface waters were fresher, with distinct vertical stratification due to salinity. Barnacle larvae were classified into early nauplii, late nauplii, and cyprid stages, and the mitochondrial COI barcode marker was sequenced to identify individual larvae of different stages. In an analysis of 164 barcode sequences, larvae belonging to Amphibalanus sp., Semibalanus balanoides, and Chthamalus fragilis were found, with Amphibalanus sp. the most abundant larval taxon overall. In the second deployment, early and late nauplii were relatively more abundant near the bottom. However, there was no obvious pattern relative to depth with cyprids, and there were no clear cross-shore distributional patterns for nauplii and cyprids. While additional deployments are necessary to corroborate these observations, the results demonstrate the feasibility of this approach. Autonomous vehicle based sampling has the potential to collect larvae of other invertebrates as well as zooplankton, and together with genetic identifications, overcomes many existing limitations and will provide valuable new insight in understanding larval distributions and transport dynamics. (C) 2015 Elsevier B.V. All rights reserved.
机译：描述了一种自动收集幼虫和环境变量（例如温度，盐度和循环）的新方法。最近开发的用于深海环境中离散生物地球化学样品收集的大体积原位泵送系统已改装并安装到自主水下航行器（AUV）REMUS 600，用于沿海水域的自主幼体和环境采样。为了评估该系统的性能，2014年3月在美国马萨诸塞州的巴扎德斯湾评估了两个垂直于海岸线（近似于9.9和11.2 km）的横断面幼虫的分布。第二个样带包括通过相对较深的通道进行的复杂采样任务，并以不连续的深度间隔进行采样。在这种部署中，近岸和地表水较新鲜，由于盐分而具有明显的垂直分层。藤壶幼虫分为早幼幼虫，晚幼幼虫和赛普勒阶段，对线粒体COI条形码标记进行测序以鉴定不同阶段的个体幼虫。在对164条条形码序列的分析中，发现了属于Amphibalanus sp。，Semibalanus balanoides和Chthamalus fragilis的幼虫以及Amphibalanus sp。总体上最丰富的幼虫类群。在第二次部署中，底部附近的早期和后期无节幼体相对较多。然而，对于塞浦路斯人来说，没有明显的与深度有关的模式，对于无节幼体和塞浦路斯人也没有明显的跨岸分布模式。虽然需要更多的部署来证实这些观察结果，但结果证明了这种方法的可行性。基于车辆的自主采样具有收集其他无脊椎动物和浮游生物的幼虫的潜力，并且与遗传识别一起克服了许多现有的局限性，并将为了解幼虫的分布和运输动态提供有价值的新见解。 （C）2015 Elsevier B.V.保留所有权利。

著录项

相似文献

  • 外文文献
  • 专利
获取原文

客服邮箱：kefu@zhangqiaokeyan.com

京公网安备：11010802029741号 ICP备案号：京ICP备15016152号-6 六维联合信息科技 (北京) 有限公司©版权所有

  • 客服微信

  • 服务号