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首页> 外文期刊>Journal of Experimental Marine Biology and Ecology >Direct measurement of dimethylsulfoniopropionate (DMSP) in reef-building corals using quantitative nuclear magnetic resonance (qNMR) spectroscopy
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Direct measurement of dimethylsulfoniopropionate (DMSP) in reef-building corals using quantitative nuclear magnetic resonance (qNMR) spectroscopy

机译：使用定量核磁共振（qNMR）光谱法直接测量造礁珊瑚中的二甲基磺丙酸二甲酯（DMSP）

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摘要

Reef building corals are among the largest producers of dimethylsulfoniopropionate (DMSP), a sulfur molecule synthesized by their endosymbiotic dinoflagellates in the genus Symbiodinium. DMSP is potentially involved in important physiological and ecological processes in corals, but investigating the functional role of this molecule requires rapid and accurate quantification techniques. Here we introduce a simple method enabling direct quantification of DMSP and one of its breakdown products acrylate using quantitative nuclear magnetic resonance (qNMR) spectroscopy. The method was tested on a range of coral genera and presents a number of advantages over currently used quantification techniques, including simultaneous and direct quantification of multiple molecules from the same extract, and rapid processing with high reproducibility enabling analyses of large numbers of samples in short time periods. The method was successfully applied to environmental samples and provides the first baseline information on diel variation of DMSP and acrylate concentrations in the coral Acropora millepora.
机译：造礁珊瑚是最大的二甲基磺丙酸二甲酯（DMSP）生产者之一，二甲基磺丙酸二甲酯是由其内生共生鞭毛虫在共生菌属中合成的。 DMSP可能参与珊瑚的重要生理和生态过程，但是要研究该分子的功能，需要快速而准确的定量技术。在这里，我们介绍一种简单的方法，可以使用定量核磁共振（qNMR）光谱对DMSP及其分解产物丙烯酸酯之一进行直接定量。该方法在一系列珊瑚属上进行了测试，与目前使用的定量技术相比，具有许多优势，包括对同一提取物中的多个分子进行同时和直接定量，以及具有高重现性的快速处理功能，可在短时间内分析大量样品时间段。该方法已成功地应用于环境样品，并提供了有关珊瑚斜纹夜蛾中DMSP和丙烯酸盐浓度的diel变化的第一个基线信息。

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    Dianne M.Tapiolas; Jean-Baptiste Raina; Adrian Lutz; Bette L.Willis; Cherie A. Motti;

  • 作者单位

    Australian Institute of Marine Science (AIMS), PMB3, Townsville MC, Townsville, QLD 4810, Australia;

    Australian Institute of Marine Science (AIMS), PMB3, Townsville MC, Townsville, QLD 4810, Australia,AIMS@JCU, ARC Centre of Excellence for Coral Reef Studies, and School of Marine and Tropical Biology, James Cook University, Townsville, QLD 4811, Australia;

    Australian Institute of Marine Science (AIMS), PMB3, Townsville MC, Townsville, QLD 4810, Australia, AIMSdPJCU, ARC Centre of Excellence for Coral Reef Studies, and School of Pharmacy and Molecular Sciences, James Cook University, Townsville, QLD 4811, Australia;

    AIMS@JCU, ARC Centre of Excellence for Coral Reef Studies, and School of Marine and Tropical Biology, James Cook University, Townsville, QLD 4811, Australia;

    Australian Institute of Marine Science (AIMS), PMB3, Townsville MC, Townsville, QLD 4810, Australia;

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  • 正文语种 eng
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  • 关键词

    Circadian rhythm; Dimethylsulfoniopropionate (DMSP); Quantitative nuclear magnetic resonance; (qNMR); Reef-building corals;

    机译：昼夜节律;丙酸二甲酯（DMSP）;定量核磁共振;（qNMR）;造礁珊瑚;

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