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首页> 外文期刊>Journal of Experimental Marine Biology and Ecology >Fluorescence and absorption characteristics of dissolved organic matter excreted by phytoplankton species of western Bay of Bengal under axenic laboratory condition
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Fluorescence and absorption characteristics of dissolved organic matter excreted by phytoplankton species of western Bay of Bengal under axenic laboratory condition

机译：在无菌实验条件下孟加拉西湾浮游植物分泌的溶解性有机物的荧光和吸收特性。

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摘要

Phytoplankton exudates are major source of dissolved organic matter (DOM) in the Sea. We examined the absorption and fluorescence characteristics of Tetraselmis spp, Cylindrotheca closterium and Odontella aurita exudates in an axenic culture experiment. The objective was to test the ability of phytoplankton to produce fluorescent DOM (FDOM) apart from protein fluorescent matter in the absence of bacteria, and if so how does the fluorescence efficiency expressed as fluorescence-absorption coefficient ratio of different FDOM components compare. Through our study, the ratio of absorption coefficients at 350 nm and 440 nm (a_(350:a_(440)) ratio) has the potential to be a unique descriptor of the growth condition of algae. It was minimum (4.12 ± 0.59, n = 14) in the growth phase for the three species. The spectral slope (S_(300-440)) indicates that higher molecular weight compounds are present in exudates when phytoplankton grows than during lag or decay. Protein (tryptophan) like fluorescence was contained in exudates throughout the life cycle. It constituted the most abundant component also, ca., ~69% of total fluorescence in Tetraselmis spp, and C. closterium, and -62% in the larger celled 0. aurita. The remaining fluorescence was made up of humic like fluorophores of the UV (two varieties), Visible and Marine types, all of which showed maxima at the end of exponential stage. The accumulated fluorescence at the close of the experiment surviving photochemical degradation was maximum for the visible humic fluorophore in Tetraselmis sp. (~187%) and for the protein like fluorophore in 0. aurita (71%).
机译：浮游植物的渗出物是海洋中溶解有机物（DOM）的主要来源。我们在无菌培养实验中检查了Tetraselmis spp，Cylindrostheca closterium和Odontella aurita渗出液的吸收和荧光特性。目的是测试在没有细菌的情况下，浮游植物产生除蛋白质荧光物质之外的荧光DOM（FDOM）的能力，如果是，则如何比较以不同FDOM成分的荧光吸收系数比表示的荧光效率。通过我们的研究，在350 nm和440 nm处的吸收系数之比（a_（350：a_（440））比）有可能成为藻类生长条件的独特描述。这三个物种在生长期最低（4.12±0.59，n = 14）。光谱斜率（S_（300-440））表明，浮游植物生长时，与滞后或腐烂过程相比，分子量更高的化合物存在于渗出物中。在整个生命周期中，渗出液中都含有类似荧光的蛋白质（色氨酸）。它也构成了最丰富的成分，约占Tetraselmis spp和C. closterium中总荧光的约69％，以及在较大的蜂窝状0. aurita中为-62％。其余的荧光由紫外线（两个变种），可见光和海洋型的腐殖质荧光团组成，所有这些都在指数级结束时显示出最大值。在实验结束时，由于光化学降解，累积的荧光对于Tetraselmis sp中的可见腐殖质荧光团最大。 （〜187％）以及金黄色葡萄球菌中的荧光团等蛋白质（71％）。

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