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首页> 外文期刊>Journal of Experimental Marine Biology and Ecology >Gene expression analysis in Mytilus chilensis populations reveals local patterns associated with ocean environmental conditions
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Gene expression analysis in Mytilus chilensis populations reveals local patterns associated with ocean environmental conditions

机译：智利Mytilus种群的基因表达分析揭示了与海洋环境状况相关的局部模式

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摘要

Marine ecosystems involve relationships between genomic interactions of marine populations with shared biogeographic ranges and the environmental conditions. These relationships, studied mainly through neutral DNA markers, are not always consistent with actual biogeographic patterns or the evolutionary history of marine species. In addition, increased information at functional genomic level from non-model species allows the study of adaptive responses in marine populations. This work reports local transcriptomic patterns in populations of the mussel Mytilus chilensis and their correspondence with oceanographic variability in southern Chile. Analysis of gene expression patterns was conducted through qPCR of seven candidate genes involved in the response to environmental stress (HSP70. HSP90), iron metabolism (Ferritin), pathogens (Mytilin B, Defensin) and oxidative stress (SOD-CuZn, Catalase) in at five study sites located in southern Chile, from Valdivia (39°56'S-73°36'W) to Melinka (43°52'S-73°44'W). Multivariate and correlation analyses were used to assess the relationship between levels of individual gene expression and site features characterized using satellite data on surface temperature, chlorophyll concentration and total suspended sediments. Two main groups of sites with differential patterns of gene expression were identified. Individuals exposed to higher temperatures showed an overexpression of HSP70, HSP90 and Ferritin genes. The expression o(SOD-CuZn and Catalase was correlated with local chlorophyll-a (i.e. food availability for mussels), although with opposite correlations. In addition, Mytilin B showed higher levels of expression in areas with higher freshwater influence. Patterns of gene expression across the region of interest suggest that spatial variability in environmental conditions induce phenotypic changes in different populations of the same mussel species. In addition, the analysis of expression patterns in candidate genes can reveal local patterns in populations where other molecular markers show no genetic structure.
机译：海洋生态系统涉及具有共同生物地理范围的海洋种群的基因组相互作用与环境条件之间的关系。这些关系主要通过中性DNA标记进行研究，并不总是与实际的生物地理模式或海洋物种的进化史相一致。此外，非模型物种在功能基因组水平上获得的信息越来越多，可以研究海洋种群的适应性反应。这项工作报告了智利南部贻贝贻贝种群的局部转录组模式及其与海洋变异的对应关系。基因表达谱分析通过qPCR对参与环境胁迫（HSP70。HSP90），铁代谢（Ferritin），病原体（Mytilin B，防御素）和氧化应激（SOD-CuZn，过氧化氢酶）的七个候选基因进行了分析。在智利南部的五个研究地点，从瓦尔迪维亚（39°56'S-73°36'W）到梅林卡（43°52'S-73°44'W）。多变量和相关分析用于评估单个基因表达水平和位点特征之间的关系，这些特征使用有关地表温度，叶绿素浓度和总悬浮沉积物的卫星数据表征。确定了两个主要的具有不同基因表达模式的位点。暴露于较高温度下的个体显示出HSP70，HSP90和铁蛋白基因的过表达。 o（SOD-CuZn和过氧化氢酶）的表达与当地叶绿素-a（即贻贝的食物供应量）相关，尽管具有相反的相关性；此外，Mytilin B在具有较高淡水影响的区域显示出较高的表达水平。研究表明，环境条件的空间变异会导致同一贻贝物种不同种群的表型变化，此外，候选基因表达模式的分析可以揭示其他分子标记没有遗传结构的种群的局部模式。

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    Gustavo Nunz-Acuna; FabianJ.Tapia; Pilar A. Haye; Cristian Gallardo-Escarate;

  • 作者单位

    Laboratorio de Biotecnologia y Cenomica Acuicola, Centra de Biotecnologla, Universidad de Concepcion, Chile,Departamento de Oceanografia & COPAS Sur-Austral, Facultad de Ciencias Naturalesy Oceanograficas, Universidad de Concepcion, P.O. Box 160-C, Concepcion, Chile;

    Departamento de Oceanografia & COPAS Sur-Austral, Facultad de Ciencias Naturalesy Oceanograficas, Universidad de Concepcion, P.O. Box 160-C, Concepcion, Chile;

    Laboratorio de Diversidad Molecular, Departamento de Biologia Marina, Facultad de Ciencias del Mar, Universidad Catolia del Norte,& Centro de Estudios Avanzados en Zonas Aridas (CEAZA), P.O. Box 117, Coquimbo, Chile;

    Laboratorio de Biotecnologia y Cenomica Acuicola, Centra de Biotecnologla, Universidad de Concepcion, Chile,Departamento de Oceanografia & COPAS Sur-Austral, Facultad de Ciencias Naturalesy Oceanograficas, Universidad de Concepcion, P.O. Box 160-C, Concepcion, Chile;

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  • 正文语种 eng
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  • 关键词

    gene expression; mytilus chilensis; adaptative response; environmental stress; thermal tolerance; sea surface temperature;

    机译：基因表达;智利的Mytilus适应性反应;环境压力耐热性海面温度;

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