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首页> 外文期刊>Journal of Experimental Marine Biology and Ecology >Cellular and biochemical parameters in the crab Carcinus aestuarii after experimentally-induced stress: Effects of bacterial injection, leg ablation and bacterial injection/leg ablation combination
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Cellular and biochemical parameters in the crab Carcinus aestuarii after experimentally-induced stress: Effects of bacterial injection, leg ablation and bacterial injection/leg ablation combination

机译：实验性胁迫后螃蟹河蟹的细胞和生化参数：细菌注射，腿部消融和细菌注射/腿部消融组合的影响

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摘要

The effects of bacterial injection, leg ablation and bacterial injection/leg ablation combination on cellular and biochemical parameters of the crab Cardnus aestuarii were evaluated. Total haemocyte count (THC), haemocyte volume, haemocyte proliferation, phenoloxidase (PO) activity in both haemocyte lysate (HL) and cell-free haemolymph (CFH) and CFH glucose levels were used as biomarkers of stress in crabs. After 24 h following bacterial injection, no significant variation in THC were observed, whereas haemocyte diameter and volume decreased significantly in injected crabs with respect to controls. Haemocyte proliferation and glucose levels increased significantly in injected crabs, whereas no significant variations in PO activity was recorded between control and injected crabs. THC increased significantly one-day after leg ablation, whereas it decreased significantly after 3 and 7 days. Haemocyte diameter and volume did not vary significantly between control and de-clawed crabs at each sampling times. In de-clawed crabs, haemocyte proliferation was significantly higher after 7 days, whereas glucose and PO activity increased significantly in CFH after 1 and 3 days. The bacterial injection/leg ablation combination caused a statistically significant decrease in both THC and haemocyte diameter and volume, whereas increased significantly glucose levels in CFH. Overall, results obtained demonstrated that stress conditions investigated in the present study affected both cellular and biochemical parameters in C aestuarii. Results also indicated that crabs can cope with differing stressful conditions modulating their immune parameters depending on the kind of stress undergone. Although preliminary, our results may explain - partially at least - the great adaptability and widespread of C. aestuarii out of its native area.
机译：评估了细菌注射，腿部消融和细菌注射/腿部消融组合对蟹类Cardnus aestuarii细胞和生化参数的影响。总血细胞计数（THC），血细胞体积，血细胞增殖，血细胞裂解液（HL）和无细胞血淋巴（CFH）和CFH葡萄糖水平中的酚氧化酶（PO）活性均用作螃蟹应激的生物标记。细菌注射后24小时后，未观察到THC的显着变化，而与对照相比，注射的螃蟹的血细胞直径和体积显着降低。在注射的螃蟹中，血细胞增殖和葡萄糖水平显着增加，而对照和注射的螃蟹之间未观察到PO活性的明显变化。腿消融术后1天，THC显着升高，而3天和7天后，THC显着下降。对照蟹和去爪蟹在每个采样时间的血细胞直径和体积没有显着变化。在去爪蟹中，7天后血细胞增殖显着更高，而CFH中的葡萄糖和PO活性在1天和3天后显着增加。细菌注射/腿部消融的组合引起THC和血细胞直径和体积的统计学显着降低，而CFH中的葡萄糖水平显着升高。总体而言，获得的结果表明，在本研究中研究的应激条件影响了南美锥C的细胞和生化参数。结果还表明，螃蟹可以应付不同的压力条件，根据所承受的压力种类来调节其免疫参数。尽管是初步的，但我们的结果至少可以部分地解释-马齿C的巨大适应性和在其原产地外的广泛传播。

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