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首页> 外文期刊>Journal of Experimental Marine Biology and Ecology >Estimating recent growth in the cuttlefish Sepia officinalis: are nucleic acid-based indicators for growth and condition the method of choice?
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Estimating recent growth in the cuttlefish Sepia officinalis: are nucleic acid-based indicators for growth and condition the method of choice?

机译：估计墨鱼乌贼墨最近的生长：选择基于核酸的生长指示剂和条件是否合适？

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摘要

A laboratory calibration study was undertaken with juvenile Sepia officinalis (80-85 g initial wet weight) to investigate the effects of different food rations and different starving intervals on RNA/dry weight (DW) ratios and RNA/DNA ratios in cephalopod mantle muscle at two different temperatures. The digestive gland index was also used as an additional indicator of recent growth. High food rations and low temperature went along with high RNA/DW ratios and high RNA/DNA ratios. Starving resulted in a linear decline in growth performance and a concomitant decrease in RNA/DW and RNA/DNA ratio, with RNA/DNA ratios representing the growth data better. RNA/DNA ratios decreased faster at higher temperatures. A fluorimetric assay for nucleic acid analysis was optimized for cephalopod mantle tissues and yielded reproducible RNA/DNA ratios with a relative variance below 10%. Thus, it may be possible to use this estimator of recently encountered feeding regime for the evaluation of mortality rates of early teuthid paralarvae to eventually support stock management. Also, log relative digestive gland weight showed a strong relationship with starving time, but, surprisingly, not with temperature. Data from the two temperatures analyzed could be combined to form a common regression line of relative digestive gland index with starving time. This indicator for recent growth might be especially suitable for large specimens with a well-developed digestive gland.
机译：对少年乌贼（80-85 g初始湿重）进行了实验室校准研究，以研究不同食物配比和不同饥饿间隔对头足纲披风肌肉中RNA /干重（DW）比和RNA / DNA比的影响。两个不同的温度。消化腺指数也被用作近期生长的另一个指标。高食物配比和低温伴随着高RNA / DW比和高RNA / DNA比。饥饿导致生长性能线性下降，RNA / DW和RNA / DNA比率随之下降，其中RNA / DNA比率更好地代表了生长数据。在较高温度下，RNA / DNA比率下降得更快。优化了一种用于核酸分析的荧光测定法，用于头足类动物的披风组织，并产生了可再现的RNA / DNA比，相对方差低于10％。因此，有可能使用这种最近遇到的喂养方式的估算器来评估早期的齿缝类幼虫的死亡率，以最终支持种群管理。此外，原木相对消化腺的重量与饥饿时间显示出很强的关系，但令人惊讶的是，与温度没有关系。可以将来自两个温度的数据进行分析，以形成饥饿时间相对消化腺指数的共同回归线。该近期生长指标可能特别适合消化腺发达的大型标本。

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